ethidium bromide is a dye that is used to stain the gel and allows the dna to be viewed under uv light.true
false
Answers
This statement is TRUE that Ethidium bromide is a dye that is used to stain the gel and allows the DNA to be viewed under UV light.
A low-molecular-weight blue dye that will swiftly flow through the gel when charged is used to add the test DNA to the gel. This gives a visual indication of the gel's development. The procedure is finished when the blue dye has traveled to the other side of the well. A running buffer known as TAE that is made up of Tris base, acetic acid, and EDTA (ethylene diamine tetra acetic acid) is applied to the agarose gel. This gives the current an electrolyte and keeps the DNA in the gel in tact.
The DNA in the agarose gel is stained with an Ultra violet fluorescent dye so that it can be captured on camera and viewed under a UV light source. Ethidium bromide, the dye, is a fluorescent substance that, when employed as a stain, inserts itself between base pairs. Due of its severe toxicity, this has a disadvantage. There are guidelines for managing this material, and it should always be handled with caution.
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Related Questions
how do I explain science in my own words
Answers
Answer:
you can say science is more important for my life
Why would having a shock-absorbing skull be a disadvantage for woodpeckers
Answers
Having a shock-absorbing skull causes the dissipation of head kinetic energy, which means woodpeckers have to work more to drill into the trunk of a tree.
How do woodpeckers absorb shock?The front of a woodpecker's skull is made of spongy bone as an adaptation to absorb or disperse shocks from its pecks, safeguarding its soft brains.
A woodpecker's skull is made up of bones that protect the brain and prevent concussions.
But having a shock-absorbing skull is a disadvantage for woodpeckers because it dissipates the kinetic energy in the head, due to which they have to work more to drill into the trunk of a tree.
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This organelle uses light energy to make sugar from carbon dioxide and water are
Answers
Answer: Chloroplast
Explanation:
Plants and some algae have chloroplasts responsible for photosynthesis. Plants undergo photosynthesis to transform light energy into chemical energy. This energy is stored in the form of sugar to support their growth and provide energy. Chloroplasts have a double membrane, with the thylakoid membrane located within the inner membrane.
Photosynthesis occurs within the thylakoid membrane, which is folded into sacs called thylakoids. Chlorophyll, a green pigment, is present in the thylakoids and it absorbs light energy to split water molecules into hydrogen and oxygen. The oxygen is released into the atmosphere while the hydrogen is used to create ATP, a molecule that stores energy. ATP and hydrogen are then utilized to make sugar, the final product of photosynthesis.
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Chloroplast
Explanation:
Chloroplast is a green pigmented organelle which some eukaryotes (such as plants and algae) have in their cells and it is in charge of photosynthesis.
Of all the mutations that occur in a population, why do only a small fraction become widespread among the population's members?
Answers
Of all the mutations that occur in a population, only a small fraction become widespread among the population's members due to a variety of factors.
Firstly, not all mutations are advantageous or beneficial to the organism. In fact, many mutations are neutral or even harmful, and as a result, they are less likely to be selected for and become widespread. Secondly, even if a mutation is advantageous, it may not spread throughout the population due to genetic drift. Genetic drift is a random process that can cause the frequency of a particular allele (variant of a gene) to increase or decrease over time, even if it is not beneficial or harmful. Therefore, even if a beneficial mutation arises, it may not necessarily be selected for and spread throughout the population due to genetic drift. Finally, the speed at which a mutation spreads through a population also depends on the size of the population, the degree of genetic diversity, and other ecological and environmental factors. In small populations, genetic drift can have a significant impact on the spread of a mutation, while in larger populations, natural selection may play a more important role.
Overall, the spread of mutations in a population is a complex process that depends on a variety of factors, including the nature of the mutation, the size and genetic diversity of the population, and other ecological and environmental factors.
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trey wrote a few steps to describe how carbon circulates between the atmosphere and living organisms step one carbon enters the atmosphere when fossil fuels are burned, step two plants absorb carbon in the air through photosynthesis , step three plant-eating animals consume the carbon stored in plants . if step two is removed , which statement best describes the effects of this missing step on the carbon cycle ? 1.more carbon is stored in the atmosphere 2.there is a decrease in the amount of carbon stored in the air 3.more carbon is stored in plants instead of the air 4.animals consume food with extra stored carbon 
Answers
Answer:
If step two is removed, which describes the effects of this missing step on the carbon cycle?
Step two states that plants absorb carbon in the air through photosynthesis. Therefore, if this step is removed, there will be a decrease in the amount of carbon stored in the plants, which will ultimately affect the amount of carbon stored in the air and in living organisms that consume these plants.
So, the best answer is:
There is a decrease in the amount of carbon stored in the air.
I Hope This Helps!
Which term means a group of organiss that have similar traits and are able to produce fertile offspring?
Answers
Answer: species
Explanation:
A promoter and a start codon are similar in that both are sequences of [nucleic acids] that are required to start important processes, and both determine the sites where the process will begin. The key differences are that the promoter is needed to start [transcription] and the start codon is needed to start [translation]. Also, the promoter is a [DNA sequence] only, and the start codon is a sequence found within a(n) [mRNA].
In what ways are a promoter and a start codon similar? In what ways are they different?
Answers
A promoter and a start codon are similar in that they both function as critical sequences of nucleic acids required to initiate essential processes.
Both the promoter and the start codon determine the sites at which transcription and translation, respectively, will begin.
However, the promoter is necessary for transcription initiation and is exclusively a DNA sequence, while the start codon is essential for translation initiation and is a sequence within the mRNA.
In summary, while both play crucial roles in starting vital biological processes, the promoter is a DNA sequence required for transcription, and the start codon is an mRNA sequence required for translation
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A property that is common to all microbial cells is _____________. evolution communication between cells differentiation genetic exchange between cells motility
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A property that is common to all microbial cells is evolution.
The term microbial cells include wide variety of micro organisms which can be bacteria, virus, fungi, algae, protist etc. The process of evolution is an important process for all the living organisms in order to survive. The evolution includes variation in genetic data that is evident in the form through mutation, transfer and reduction.
Communication between cells is common in bacteria and is a well-researched topic by scientists. The term for the process is quorum sensing and is responsible for biofilm formation, which causes problems in health and other industries. Motility and genetic exchange is not common in various organisms.
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Moving a limb toward the body is _________, whereas moving a limb away from the body is ______________.
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Moving a limb toward the body is known as adduction, whereas moving a limb away from the body is known as abduction. Adduction is the movement of an appendage or body section towards the midline of the body.
This is distinct from abduction, which is the opposite motion, where the limb or extremity is moved away from the body's midline.Adduction is the opposite of abduction, which involves movement away from the body's midline. Examples of adduction include bringing the arm closer to the body or the fingers closer to the palm. What is Abduction?Abduction is the opposite motion of adduction.
This movement occurs when a limb is raised or moved laterally away from the body's midline. This is the motion that raises the arms out to the sides of the body to the horizontal position.What is the difference between adduction and abduction?Abduction and adduction are terms used to describe movements towards or away from the midline of the body. Adduction is the movement of a body part towards the body's midline, whereas abduction is the movement of a body part away from the body's midline.
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Suppose you are helping scientists conduct a study of a population of Rainbow Trout, which is a fish species that lives in California lakes. The scientists initially capture 50 Rainbow Trout from a lake and tag all of them. After 30 days, they capture 75 Rainbow Trout and count 15 tags.
Find the estimated total number of Rainbow Trout in the lake.
__________ Rainbow Trout.
(Hint: Use the equation: N = (M x T)/R)
Answers
Answer:
250
Explanation:
N = (M*T/R)
substitute
N=(50*75/15)simplify
N=(3750/15)N=(250)Metal are malleable and ductile. Explain on the basis of the electron sea level?
Answers
Metallic Bonding is restricted to metals only. In the metallic bonding, There is a electrostatic force of atrraction between the metal ions (which are positive)
and the electrons(which are negative).
The metal ions are arranged in a lattice pattern which is the regular repetitive structure.
_____________________________________Electron Sea Level:Every metal atom donate one or two valence electrons, so due to the BULK presence of the electrons, It results in a formation of sea of electrons. So the valence electrons don't just stay on their own atom, they roam around the whole metal. They are just like a sea of electrons. This is why it is called the electron sea model. basically metal ions are present as a iceberg or island in the freely floating sea of electrons.
_____________________________________How is Sea of electron related to the malleable and ductile property?The sea of electron works as a electron GLUE, That means the metal ions are strongly ATTRACTED to the electron, so When apply pressure on the metals they DONT BREAK that means they are not brittle but malleable and ductile. This is because of the moving electrons and because the cations slide easily past each other, The ability of the atoms to roll over each other into new positions without breaking the metallic bond is because of the electron glue.
_____________________________________IMPORTANT STATEMENTSBrittle; Hard but breakable due to absence of sea of electron.
Malleable and Ductile; Transformed into sheets and thin wires so they are somewhat ELASTIC.
In the diagram below, label parts A-L next to the words in the structures of photosynthesis box below.
Answers
A leaf; B a cuticle; C a layer of skin; D a layer of mesophyll cells Chloroplast E Nucleus F See the entire response.
What characteristics does the nucleus have?
Properties. The cell's genetic material is housed in the nucleus, a membrane-bound organ that also regulates cellular growth and reproduction. It is typically the cell's most noticeable organelle. The nuclear envelope is the framework that encloses it. This membrane divides the cytoplasm from the nucleus's contents.
What functions does a cell's nucleus serve?
DNA-containing chromosomes are housed in the nucleus. DNA contains instructions for cell development, growth, and reproduction as well as genetic information. The chromosomes of a cell are arranged into lengthy, tangled structures called nucleosome while the cell is "resting," or not dividing.
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One example of an object or substance the dose not have the property of color
Answers
What type of stress occurs when a rock mass is pushed in parallel and opposite directions?
Answers
Answer:
Shear stress
Explanation:
Shear stress is a type of stress that occurs when a rock mass is pushed in parallel and opposite directions.
which event is mostly responsible for increasing the opportunity for animals to develop more complex traits?
A. increase in genetic material (DNA)
B. jaws
C. bilateral symmetry
D. spinal column
Answers
Increase in genetic material (DNA) event is mostly responsible for increasing the opportunity for animals to develop more complex traits. The correct answer is option A.
The evolution of life on earth is marked by an increase in the amount of genetic material in living organisms. This increase in genetic material has enabled animals to develop more complex traits and adapt to a wider range of environments.
The gradual accumulation of genetic changes over time, as well as the movement of organisms into new environments, has led to the emergence of new species with unique physical and behavioral characteristics.
In many cases, these new species have been able to survive and thrive in their new environments because of the complex traits they possess, which allow them to compete for resources and avoid predators more effectively.
The correct answer is option A.
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Which of the following statements about greenhouse gases and the greenhouse effect is true?
A.)Greenhouse gases are transparent to longwave thermal radiation but absorb shortwave solar radiation.
B.)Greenhouse gases reradiate thermal energy as longwave radiation in all directions.
C.)The greenhouse effect on Earth is caused entirely by a single greenhouse gas, carbon dioxide.
D.)The greenhouse effect causes energy to be reflected from the surface of the Earth back into space.
Answers
The correct statement about greenhouse gases and the greenhouse effect is greenhouse gases reradiate thermal energy as longwave radiation in all directions, option B is correct.
Greenhouse gases, such as carbon dioxide, methane, and water vapor, have the ability to absorb and re-emit infrared radiation (longwave radiation) in the Earth's atmosphere. When sunlight reaches the Earth's surface, it warms the surface, which then emits infrared radiation.
Greenhouse gases absorb a portion of this outgoing longwave radiation and re-emit it in all directions, including back toward the Earth's surface and into space. This trapping and re-radiation of heat by greenhouse gases create a natural phenomenon known as the greenhouse effect, option B is correct.
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the towns current population is 4790 people. the population is expected to increase by 7.0% over the never 5 years. based on the current per capita consumption (12,000 m^2), how much land must be cleared to accommodate population growth
Answers
Approximately 4.02 hectares of land would need to be cleared to accommodate the projected population growth over the next 5 years based on the given parameters.
To calculate the amount of land that must be cleared to accommodate population growth, we need to determine the increase in population and multiply it by the per capita consumption of land.
Given that the current population is 4790 people and it is expected to increase by 7.0% over the next 5 years, we can calculate the population growth as follows:
Population growth = Current population \(\times\)Population growth rate
= 4790 \(\times\) 0.07
= 335.3 (rounded to the nearest whole number)
Therefore, the population is projected to increase by approximately 335 people.
Now, we can calculate the amount of land that needs to be cleared to accommodate this population growth. Assuming a per capita consumption of 12,000 \(m^2\) (or 0.012 hectares), we can calculate the land required as:
Land required = Population growth \(\times\) Per capita consumption
= 335 \(\times\) 0.012
= 4.02 hectares
Hence, approximately 4.02 hectares of land would need to be cleared to accommodate the projected population growth over the next 5 years based on the given parameters.
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Which statement might explain why there are so many variations of a particular phage type, eg. coliphages, for E.coli? (Check all that apply.) As E cow species evolved and differentiated into different strains the phages had to change in order to infect the different strains Oferent strains of E coll developed as a result of aptibiotic exposure, and the page had to evolve along with the host Every time new pages burst out of 6 host cell the new viruses are genetically different from the original infectious phoge
Answers
The third statement "Every time new phages burst out of a host cell the new viruses are genetically different from the original infectious phage" is correct.
The given question is asking for the statements that explain why there are so many variations of a particular phage type like coliphages for E.coli. So, let's see which statement is correct: Every time new phages burst out of a host cell, the new viruses are genetically different from the original infectious phage. It helps to explain why there are so many variations of a particular phage type, such as coliphages, for E.coli.
Therefore, the third statement "Every time new phages burst out of a host cell the new viruses are genetically different from the original infectious phage" is correct and explains why there are so many variations of a particular phage type.
It can be concluded that the answer is option C.
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Need some help with biology!!
Answers
Answer:
a. X chromosome
B. 1-1, 11-2, 11-3, 111-4
4. 0%
Explanation:
Which of the following is the correct flow of nutrients?
A. Cloud of nutrients tiny microbes caddisfly fish
B. Fish- tiny microbes caddisfly cloud of nutrients
C. Fish caddisfly tiny microbes cloud of nutrients
D. Cloud of nutrients caddisfly tiny microbes fish
Answers
Answer: d
Explanation: fishy fishy fishy
The graph below shows the amount of CO2 (carbon dioxide) in the atmosphere throughout Earth’s history.
Which of the following claims is supported by the evidence in this graph?
A
The amount of CO2 in the atmosphere is currently decreasing at an extremely fast rate.
B
High amounts of CO2 in the atmosphere correspond to cold periods in Earth’s history, called ice ages.
C
The amount of CO2 in the atmosphere in 2018 was significantly higher than any other time in the past 800,000 years.
D
The amount of CO2 in the atmosphere from 1800 to 2018 increased at a slower rate than at any other point in the past 800,000 years.
Answers
Explanation:
is there a graph to be shown
When will a fossil be used in finding the absolute age of rocks?
Answers
The most helpful of these methods is radiometric dating because it is the only one that can determine the age of objects older than a few thousand years.
What fossil be used in finding the absolute age of rocks?Researchers use some kind of clock to ascertain the date when a rock or fossil was generated in order to calculate its age. In order to reliably date historic events, geologists frequently utilize radiometric dating techniques, which are based on the natural radioactive decay of specific elements like potassium and carbon.
Utilizing radioactive materials in the rock, absolute age can be determined. If specific fossils are found in the same rock, their age is presumed to be the same, with the absolute age determined by radioactive materials.
Therefore, during the radiometric dating method. In order to determine an object's absolute age, geologists employ a range of methods, including as radiometric dating, tree rings, ice cores, and seasonal sedimentary deposits known as varves.
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The thin layer of liquid and frozen water that now surrounds our planet is called the lithosphere
Answers
The given statement, "The thin layer of liquid and frozen water that now surrounds our planet is called the lithosphere" is not correct.
The thin layer of liquid and frozen water that now surrounds our planet is called hydrosphere. Hydrosphere is the part of the earth's surface where water is found, comprising of oceans, rivers, seas, lakes, groundwater, and other water bodies. It includes all water, ice, and vapor on the planet that is circulated by the hydrologic cycle, which includes evaporation, precipitation, and condensation.
In contrast, the lithosphere is the rigid outer part of the earth, consisting of the crust and upper mantle. It is also called the geosphere and is made up of rocks and minerals. It is the solid, rocky, and non-living part of the earth.The terms "content loaded" are not relevant to this question.
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What are some benefits of meiosis? and mitosis?
Why does it make sense that our sex cells use meiosis?
How might mutations in dna affect an organism?
What do mutations cause?
What are everyday things that can cause mutations?
Answers
Answer:
It comes from the woman’s Fallopian tube after fertilization
Explanation:
After the sperm successfully reaches to the woman’s egg it goes to the Fallopian tube and so on
which phrases describe non-foliated rocks
Answers
Answer:
Non-foliated rocks have grains that are arranged randomly, and grains that do not split into layers.
Explanation:
I'm not sure what the question was asking, but I just gave phrases that described non-foliated rocks. I hope that helps?
how do you define a cladogram
Answers
Answer:
A cladogram is a branching diagram showing the cladistic relationship between a number of species.
Nuclear energy is a non-renewable energy source.
True
False
Answers
Explanation:
Non renewable energy resources are resources that can be used up quickly and cannot regenerate .
Answer:
True True
Explanation:
True not FalsePLS HELP ITS EARTH SCIENCE
Landforms can be shaped by different types of weathering. Which of the following occurred because of chemical weathering?
A. Underground rocks react with acidic groundwater and slowly dissolve, forming a cave.
B. a large section of rock is formed into an arch.
C. The rocks found near a river are rounded.
D. Small cracks in a rock get larger during winter.
Answers
Answer:
A
Explanation:
This produces a weak acid, called carbonic acid, that can dissolve rock.
Differences in domesticated animals over relatively short periods of time most likely occur through...
a) evolution
b) artificial selection
c) experimental selection
d) adaptation
e) natural selection
Answers
The differences in domesticated animals over relatively short periods of time most likely occur through artificial selection. Artificial selection is the process of humans intentionally selecting and breeding certain traits in domesticated animals to produce desired characteristics, such as larger size or higher milk production.
This differs from natural selection, which occurs in the wild and is based on survival of the fittest. Evolution, adaptation, and experimental selection may also play a role, but artificial selection is the most significant factor in the rapid changes seen in domesticated animals.
Artificial selection is the identification by humans of desirable traits in plants and animals, and the steps taken to enhance and perpetuate those traits in future generations.
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How many calories per day would a 5 month old, 7 kg infant need based on 108 calories per kg? Round to the nearest whole calorie. A recipe contains a total of 100 grams of fat. The recipe feeds 4 people. How many grams of fat is in each serving?
Answers
A 5-month-old, 7 kg infant would need 756 calories per day.
Each serving of the recipe would contain 25 grams of fat.
To calculate the number of calories per day that a 5-month-old infant would need based on 108 calories per kg, we can use the infant's weight of 7 kg:
Calories per day = Weight (kg) x Calories per kg
Calories per day = 7 kg x 108 calories/kg
Calories per day ≈ 756 calories
Therefore, a 5-month-old, 7 kg infant would need approximately 756 calories per day.
To determine the number of grams of fat in each serving of a recipe that contains a total of 100 grams of fat and feeds 4 people, we divide the total fat by the number of servings:
Grams of fat per serving = Total grams of fat / Number of servings
Grams of fat per serving = 100 grams / 4 servings
Grams of fat per serving = 25 grams
Therefore, each serving of the recipe would contain 25 grams of fat.
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