Explain the contribution of antigenic determinant in false negative and false positive tests

Answers

Answer 1

Answer:

Contribution of antigenic determinant in false negative -: Popular causes of false-positive ELISA include: flu vaccine administration, presence of HLA-DR antibodies in women with multigravity, presence of rheumatoid factor. Contribution of antigenic determinant in false positive tests -: Positive RPR exam, hypergammaglobulinemia and autoimmune hepatitis (e.g. multiple myeloma).

Explanation:

Much attention has been paid to the specificity and sensitivity of this assay since regular ELISA screening of blood donors for anti-HIV antibodies was implemented. It was found that inadequate coating of the microtitre plates with HIV-antigen could lead to false negative results. It is important that the ELISA test kit manufacturers use control systems that guarantee adequate antigen coating.


Related Questions

Dizziness, pale and ashen colored skin, pain and pressure in the jaw or chest are all signs and
symptoms of:
A. A seizure
B. A stroke
C. A heart attack
D. An aneurism

Answers

The correct answer would be C.) a heart attack

who is at greatest risk of duchenne muscular dystrophy

Answers

Young boys are at great risk of Duchenne muscular dystrophy.

Duchenne muscular dystrophy (DMD) which is a genetic condition and it is characterized by progressive weakening of voluntary muscles. This disease  worsens more rapidly than other types of muscular dystrophy. And it is also the most common form of muscular dystrophy. It has been estimated that one out of every 5,600 to 7,700 males between 5 and 24 years of age has DMD.

The symptoms of DMD begin in very early childhood, and those with DMD usually die in early adulthood.

Symptoms of Duchenne muscular dystrophy are as follows:

1)Frequent falls

2)Difficulty rising from a lying or sitting position

3)Trouble running and jumping

4)Waddling gait

5)Walking on the toes

6)Large calf muscles

7)Muscle pain and stiffness

8)Learning disabilities

9)Delayed growth

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A student is planning an investigation of the effect of population size on an ecosystem. She plans to use a five-gallon fish tank with ten goldfish for her investigation. How should the student perform her investigation? A. She should limit the amount of food given and observe the results. B. She should add a predator fish to the tank and observe the results C. She should continue to add more goldfish to the tank and observe the results D. She should change the temperature of the water in the tank and observe the results.​

Answers

Answer:

C

Explanation:

Because the student is observing the effects on the population, so it would make more sense to add more fish to the tanks to see how they would get effected.

A student is planning an investigation of the effect of population size on an ecosystem, so she should continue to add more goldfish to the tank and observe the results, as in Option C.

What is the population size of an ecosystem?

In the student's investigation, the goal is to observe the effect of population size on an ecosystem using a fish tank with goldfish. The student has already decided to use a five-gallon fish tank with ten goldfish for the experiment, so by adding more goldfish to the tank, the student can observe the effect of population size on the ecosystem. As the population of goldfish increases, the competition for resources such as food, oxygen, and space will also increase. This can lead to changes in behavior.

Hence, she should continue to add more goldfish to the tank and observe the results, as in Option C.

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What do Phenylketonuria, Cystic Fibrosis, and Sickle Cell Anemia have in common?

Answers

Answer:

Phenylketonuria, Cystic Fibrosis and Sickle Cell Anemia, these all are disorders caused by hereditary mutation.

Hope it helps!

What type of tissue surrounds the slip disc with age epithelial tissue?

Answers

Answer:

It is Cartilage.

PLEASE HELP! huntington’s disease (HD) is prevalent South Africa. Most of the families with HD have Dutch or British ancestry, which suggests they inherited the European HD allele. HD is believed to have first arrived in South Africa more than 300 years ago when Dutch colonists settled into the Western Cape in the 1600s. Thus, the prevalence of HD in South Africa is the result of the founder effect.

Mutations in the HTT gene cause Huntington’s disease. The HTT gene provides instructions for making a protein called huntintin. This protein plays a role in nerve cells in the brain. The HTT mutation that causes HD involves a DNA segment known as a CAG trinucleotide repeat. This segment is made up of a series of three DNA building blocks that appear multiple times in a row. Normally, the CAG segment is repeated 10 to 35 times within the gene. In people with HD, the CAG segment is repeated 36 to more than 120 times. An increase in the size of the CAG segment leads to the production of an abnormally long version of the huntintin protein. This causes the dysfunction and eventual death of neurons in certain areas of the brain. The more repeats, the more serious the disease is.

HD is inherited in an autosomal dominant pattern. There is both an adult onset (after reproductive age) and a juvenile onset pattern. People with adult onset typically have 40 to 50 repeats, while people with the juvenile form tend to have more than 60 repeats. Individuals who have 27 to 35 repeats do not develop HD, but they are at risk of having children who will develop the disorder. As the gene is passed on to offspring, the size of the repeat may lengthen into the range associated with HD.

Predict what will happen to the allele frequencies for Huntington’s disease in South Africa if there were a new mutation to the HTT gene that causes a reduction to the CAG trinucleotide repeat. Justify your prediction with evidence. Your response should include links to the sources of information gathered.

Answers

Answer:

If there were a new mutation to the HTT gene that causes a reduction to the CAG trinucleotide repeat, the allele frequency for Huntington's disease would decrease in South Africa. HD is caused by too many repeats of the CAG segment causing an abnormally long version of the huntintin protein which becomes deadly. so if the number of repeats is reduced then the person is more likely to not have Huntington's disease. If they do still develop Huntington's disease, it will be more likely to be adult onset and they will still have an opportunity to reproduce offspring which may carry the trait for reduced CAG.

What is food hygeine​

Answers

Answer:

Food hygiene is an essential aspect of food safety. It refers to the processes that directly involve food – including storage, preparation, and cooking. Good practices in these areas ensure that people receive food that's safe and as described.

What way does air move ?Air ranges in which two pressures ?

A)Vertically ;L to H

B)Horizontally;H to L

C)Vertically;H to L

D)Horizontally;L to H

Answers

B.) horizontally; H to L

I just did the text and the obvious answer is B

B) Horizontally; H to L :)

The main purpose of a multiple cloning site is:

1. To keep the plasmid from leaving the nucleoid region.

2. To ensure the plasmid is copied many times in the bacterial cell.

3. To provide a location where DNA can be inserted into a plasmid.

4. To make it possible to eliminate bacteria that have not been genetically modified.

5. To ensure that each daughter cell receives a copy of the plasmid during cell division.

Answers

The main purpose of a multiple cloning site is: (3)To provide a location where DNA can be inserted into a plasmid.

A multiple cloning site is a sequence in the DNA of an organism that consists the cut palindromic site for many restriction enzymes. This region is highly exploited in laboratories to insert DNA into the vector for its transmission into the host.

Plasmid is the closed circular double-stranded DNA molecule. It is particularly present in bacterial cells. It is an autonomously replicating genetic material i.e., it can replicate on its own. Plasmids are largely used as vectors for gene transfer.

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Which is part of the cell theory

Answers

Answer:

The first part states that all organisms are made of cells. The second part states that cells are the basic units of life. These parts were based on a conclusion made by Schwann and Matthias Schleiden in 1838, after comparing their observations of plant and animal cells.

Explanation:

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In the lock-and-key model of enzyme action, the enzyme active site is thought of as ________.

Answers

Answer:

Rigid and non flexible surface

Explanation:

Enzymes are catalyst that speeds up rate of chemical reactions, they bind to a substrate which are known as reactant substrate to speed up rate of chemical reaction and to unlock the key.

The enzyme is said to be rigid and non flexible fitting perfectly to the surface of the substrate where the reaction takes place. Fitting perfectly enable the enzyme to perform its action on the substrate unlocking the key and activating the action in the molecule.

what is the force that moves the continents?
Conduction
Convection
Radiation
Conveyor belt

Answers

Answer:

Convection currents

when did human activity affect a few species and cause them to trophic cascade

Answers

Human activity has caused trophic cascades in several species, with one notable example being the reintroduction of gray wolves to Yellowstone National Park in 1995.

Prior to their reintroduction, the park's ecosystem had suffered due to the absence of wolves, as their absence had allowed the elk population to grow unchecked, resulting in overgrazing of vegetation and a decline in biodiversity.

Once the wolves were reintroduced, they began to prey on the elk, which reduced the elk population and allowed vegetation to recover. As a result, the habitat of other species, such as beavers, songbirds, and even fish, improved, as they were able to utilize the newly regenerated vegetation.

This is a classic example of a trophic cascade, where the removal or addition of one species can have a cascading effect on the rest of the ecosystem.

Human activities such as hunting, deforestation, and pollution have disrupted many ecosystems, causing trophic cascades that can have far-reaching consequences for biodiversity and ecosystem functioning.

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The complete question is:

When did human activity affect a few species and cause them to trophic cascade?

The signs used depend on the species being surveyed and include the presence of runways and tracks on *
1 point
(a). soft substrates
(b) hard substrate
(c) dry mud
(d) rocks

Answers

Answer:

(a). soft substrates

Explanation:

Mobile organisms can be measured by both direct and indirect methods. Direct methods may involve observing the actual behavior (e.g., scoring via timing). Moreover, indirect methods are those where the technique used depends on the species being examined, collecting information such as through recording of tracks in the soft substrate (e.g., mud, sand, snow), presence of fur/feathers, droppings (mammals), etc. For example, indirect observation of insects may involve the determination of exoskeletons, sounds, etc.

Why is it said that natural selection acts on phenotypes rather than on the genetic material of organisms?

Answers

Answer:

It's because an organism's phenotype, or physical traits, are what truly decide whether it can reproduce and pass on its genotype to the following generation.

Because the phenotype of an organism represents the alleles that are actually expressed in that organism, natural selection affects that phenotype.

Explanation:

LESSON SUMMARY: EXIT TICKET
You work for NASA, and have discovered an alien threat.
Apply your knowledge of the advantages and
disadvantages of asexual and sexual reproduction.
NASA has captured an alien and had time to study
the alien's DNA, and how their body works. They
used this information to come up with a special
virus to harm and make only aliens sick. So it
will not affect humans, but it will make aliens
sick, and eventually kill them while not alerting
the population of human beings that aliens are among us. Which type of alien
reproduction would this virus be most effective on (Pick 1 and explain why)
1)Asexually reproducing aliens 2)Sexually reproducing aliens 3) Both

LESSON SUMMARY: EXIT TICKETYou work for NASA, and have discovered an alien threat.Apply your knowledge

Answers

Asexually reproducing aliens would be more susceptible to the NASA-created virus. Option A is correct.

Which type of alien reproduction would this virus be most effective on?

This is due to the fact that asexual reproduction creates genetically identical offspring through the process of mitosis without the transfer of genetic material from one person to another. Because of this lack of genetic diversity, any mutation or genetic change caused by the virus would have an impact on the entire population. On the other hand, through meiosis and fertilisation, sexually reproducing aliens create offspring with distinctive genetic material. Due to the fact that different people would react to the virus differently due to genetic diversity, it would be more challenging for the virus to infect the entire population. As a result, the virus would spread more readily and have a greater effect on the asexually reproducing aliens.

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black footed ferrets are native to the great plains. which factor would decrease an area's carrying capacity for these ferrets?

A. decreased number of diseases
b. decreased competition for shelter
c. decreased availability of food
d. decreased number of predators

Answers

Answer:

c

Explanation:

i think might be b but most likely c

Explain the process of the Greenhouse Effect. How does it happen?

Answers

The greenhouse effect is a natural process that warms the Earth’s surface. When the Sun’s energy reaches the Earth’s atmosphere, some of it is reflected back to space and the rest is absorbed and re-radiated by greenhouse gases.

Greenhouse gases include water vapour, carbon dioxide, methane, nitrous oxide, ozone and some artificial chemicals such as chlorofluorocarbons (CFCs).

The absorbed energy warms the atmosphere and the surface of the Earth. This process maintains the Earth’s temperature at around 33 degrees Celsius warmer than it would otherwise be, allowing life on Earth to exist.

Enhanced greenhouse effect

The problem we now face is that human activities – particularly burning fossil fuels (coal, oil and natural gas), agriculture and land clearing – are increasing the concentrations of greenhouse gases. This is the enhanced greenhouse effect, which is contributing to warming of the Earth. Greenhouse effect

Step 1: Solar radiation reaches the Earth's atmosphere - some of this is reflected back into space.

Step 2: The rest of the sun's energy is absorbed by the land and the oceans, heating the Earth.

Step 3: Heat radiates from Earth towards space.

Step 4: Some of this heat is trapped by greenhouse gases in the atmosphere, keeping the Earth warm enough to sustain life.

Step 5: Human activities such as burning fossil fuels, agriculture and land clearing are increasing the amount of greenhouse gases released into the atmosphere.

Step 6: This is trapping extra heat, and causing the Earth's temperature to rise.

Explanation:

The greenhouse effect is a natural process that warms the Earth's surface. When the Sun's energy reaches the Earth's atmosphere, some of it is reflected back to space and the rest is absorbed and re-radiated by greenhouse gases. ... The absorbed energy warms the atmosphere and the surface of the Earth.

10 (a) Explain what is meant by translocation in plants.[4]
(b) Describe how systemic pesticides may be used to control a named pest.[4]
(c) Discuss the advantages and disadvantages of using chemicals to control pests.[7]


11 .For a named farm animal (not poultry):
(a) draw a labelled diagram to show the structure of the female reproductive system. [9]
(b) discuss the advantages of using Artificial Insemination (Al) compared with the natural service of the female animal. [6]​

Answers

Translocation in plants describes the transfer of substances from one area of the plant to another, including water, minerals, and sugars.

Translocation in plants, and artificial insemination

Translocation in plants describes the transfer of substances from one area of the plant to another, including water, minerals, and sugars. The vascular system of the plant, which comprises the xylem and phloem, is where this movement takes place. Phloem transports sugars and other organic molecules from the leaves to other parts of the plant, whereas xylem is in charge of moving water and minerals downward from the roots to the leaves.

Systemic pesticides are substances that can be sprayed on a plant's soil, leaves, or seeds and then absorbed by the vascular system of the plant to kill pests that feed on it. Neonicotinoid insecticides, for instance, can be used to manage aphids in crops.

Artificial insemination (AI) has benefits over natural insemination for female animals, including the use of superior genetics without the need to physically transfer or lodge a male animal. Without the possibility of inbreeding or genetic abnormalities that can occur with natural selection, AI enables the selection of the finest genetic features, like as milk production or disease resistance.

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please help me on this it's due tonight ​

please help me on this it's due tonight

Answers

Answer:

To better understand each other and not make any mistakes in their work because of a mistranslation.


Which of the following statements about planetary satellites is true?

Which of the following statements about planetary satellites is true?

Answers

Answer:

C

Explanation:

How do the zones in a school of fish benefit them?

A. This allows them to move more quickly when being attacked by a predator.

B. This allows them to move in unison, so that when one moves in a zone every zone shifts.

C. This helps them filter feed.

D. This allows them to protect young in the center of the group.​

Answers

A) This allows them to move more quickly when being attacked by a predator.

This is because, when fish stick together it helps the fish avoid predators by relying on eachothers eyes.

Answer: B. This allows them to move in unison, so that when one moves in a zone every zone shifts.

Explanation:

. Mushroom is under............
i. Genus
ii. Donail
iii. Taxonomy
iv. Species​

Answers

Answer:

Mushrooms are classified under the categories of "Genus" and "Species" in taxonomy. The genus refers to a group or category that includes closely related species, while the species represents a specific type or kind of organism. So, the correct options for mushrooms would be "i. Genus" and "iv. Species."

Name Why do you think some people are against genetic engineering?

Answers

genetic engineering need not involve overriding anyone's autonomy

Which of the following is the best example of an adaptation that helps an organism survive in its environment?A. Green coloring on a lizard living one red and brown rocksB. A thick coat of fur on an animal that lives in the desertC. A long root system in a desert plantD. Thin, delicate leaves on a plant that lives in the tundra

Answers

An adaptation that helps an organism survive in its environment would be an adaptation that helps this organism get food/water, hide from predators, not loose too many water or nutrients, etc.

A green lizard living in red and brow rocks would cause a great contrast between the colors, putting the lizard in evidence and making it easier for predators to find it. Therefore, A is incorrect.

The adapted leaves of plant from the tundra will be small and waxy, but not too thin as it will try to keep more water inside it than being lost in transpiration. Therefore, D is also incorrect.

An animal that lives in the desert can't have a thick coat of fur because it will make the animal be super heated, causing loss of water that is so important to keep in a desert. So B is incorrect too.

In deserts that are no water easily available, so a plant can have a long root system in order to get water from deep below the surface of the ground. Therefore, the correct answer is C. A long root system in a desert plant

which of the following most closely resembles cellular respiration

Answers

Logs burning in a campfire are the circumstances that most mimic cellular respiration. This is due to the fact that both processes release energy and depend on oxygen to continue. The body uses cellular respiration, a series of metabolic events, to liberate energy from food.

what is respiration?

This is the process of breathing; the taking in and exhaling of air. Biology. the whole of the physical and chemical mechanisms that transport oxygen to tissues and cells and release carbon dioxide and water during oxidation in an organism.

what does metabolic events?

It is absolutely necessary for an organism to preserve its life for a process known as metabolism to take place in its body. These activities, which include anabolism and catabolism, are also known as metabolic processes.

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Describe the three patterns of communication​

Answers

The three patterns of communication include:

Verbal CommunicationThe most evident kind of communication would appear to be verbal communication. It makes use of spoken language, either in-person or over the phone. Although most encounters need verbal communication, there are other nonverbal indicators that can add to the meaning of the words themselves. A more sophisticated message is conveyed when spoken language is combined with nonverbal communication.

Non-verbal CommunicationThe use of body language might reveal some information about a speaker's word choice. It can reflect sarcasm, complacency, dishonesty, or sincerity. Body language, including the speaker's posture, hand gesture, facial expressions, and even look, is a common way to communicate these things. A untidy speaker with wrinkled clothes and slouching posture, for example, might convey a lack of assurance or knowledge. Visual Communication Signs, maps, and drawings, as well as color and graphic design, are all examples of visual forms of communication. These frequently support verbal communication and aid in proving a point. Visual aids can aid in the presentation of the message by helping the speaker remember key points, providing the audience with something to look at, and more.

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A piece of alien DNA cannot multiply itself in the progeny cells of the organism because:

a. It has no the sequence called Origin of replication (ori) needed for starting replication.

b. A piece of DNA will be denatured and damaged soon as it has no stability.

c. A piece of DNA is easily unwound and the H bonds between nitrogen bases are broken.

d. Both A & B.​

Answers

I think the answer is A . It has no the

What is the general structure and function of epithelial tissue?

Answers

Answer:

Epithelial tissues are widespread throughout the body. They form the covering of all body surfaces, line body cavities and hollow organs, and are the major tissue in glands. They perform a variety of functions that include protection, secretion, absorption, excretion, filtration, diffusion, and sensory reception.

Intergrated Drilling services helps adnoc increase its drilling efficiency by what percentage?

Answers

Answer::ADNOC Drilling has also delivered over 100 integrated drilling services (IDS) wells since 2018, achieving an efficiency improvement of more than 35%, which resulted in over $170 million (AED624 million) in savings.

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Dena bought 4 stuffed animals and 7 toy trains for $75. At the same prices, Nico bought 6 stuffed animals and 5 toy trains for $74. What is the price of a stuffed animal? the process of estimating revenues and costs of alternative actions and comparing them to the status quo is called calculate the work for the expansion of co2 from 1.0 to 3.0 liters against a pressure of 1.0 atm at constant temperature. pricing is: question 24 options: a) normally a government-regulated variable. b) one of the 4 ps of the marketing mix. c) considered to be a part of promotion. d) totally based on the cost of products. in her research, andrea found a correlation of .49 between illegal drug use and levels of adolescent delinquency. this correlation is ________ and ________. Use the properties of equality to solve the equation 3x +2 - x = 11x + 9 Share how the changes of price for an elastic good and an inelastic good you use regularly has affected your purchasing and lifestyle. An IV solution of 110 mL at a drip rate of 5 gtt/min using a tubing factor of 10 gtt/mL has been ordered to be initiated at 0200. Calculate the infusion time. (Round your answer to the nearest tenth of an hour.) Check all that apply. pi/4 is the reference angle for:A.15pi/4B.19pi/4C.12pi/4D.7pi/4 a homeowner's policy not only covers the persons named in the policy but also family members who live in the insured's home. question 16 options: true false Let S={[8 -2 6+ ]: ab oed} a, (a) Prove that S is a subspace of Mus(R) by verifying that S is closed under addition and closed under scalar multiplication (b) Find a basis fo? (100 points) create a plan for the prevention of identity theft from the time a child is 10 years old to 70 years old. be very specific. Assume that the height of your cylinder is 6 inches. Consider A as a function of r, so we can write that as A(r)=2r2+12r. What is the domain of A(r)? In other words, for which values of r is A(r) defined? Which water system is better The heat pump or the groundwater system? Cost of maintenance of production tools and writing procedure and instructions are examples of which of the following cost categories for quality management? appraisal cost replacement cost external failure cost internal failure cost prevention cost The number of employees for a certain company has been decreasing eachyear by 8%. If the company currently has 630 employees and this rate continues, find the number of employees in 10 years. Please help me to solve this question as soon as possible what's the area of the quadrilateral 5 ports Use absolute value to express the distance between -12 and -15 on the number line. |-12 - (-15) = -27 The diameter of the wheel of a unicycle is 0.7m. Dan tries to ride the unicycle and manages to go in a straight line for 28 full revolutions before falling off. How far did Dan manage to cycle in metres? Give your answer to 1 DP.