how many nucleotides long is the guiding region of the sgrna?

Answers

Answer 1

The guiding region of the sgrna is twenty nucleotides long.

The length of the most widely utilised gRNA is approximately 100 base pairs. Any genomic region that is complementary to that sequence can be targeted by the CRISPR Cas9 system by changing the 20 base pairs near the 5' end of the gRNA.

A guide sequence domain, or gRNA in our study, is intended to be present at the 5′ end of sgRNA and to be complementary to the target sequence.

CRISPR-Cas9 locates and accurately chops target protospacer DNA by following the guidance of a 20 nucleotide RNA (gRNA), 3 base pairs upstream of a PAM (Protospacer Adjacent Motif).

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Related Questions

Transporters, in contrast to channels, work by Group of answer choices a gating mechanism. filtering solutes by charge. filtering solutes by size. specific recognition of transport substrates.

Answers

Transporters work by specific recognition of transport substrates.

Transporters work by specific recognition of transport substrates. They bind to a specific solute and undergo a conformational change to move the solute across the membrane. This is in contrast to channels, which allow for the passive movement of ions or molecules down their concentration gradient without any specific binding or recognition.Binding refers to the process of two or more molecules or particles coming together and forming a stable complex. Binding can occur between molecules of the same type or different types, and it can be reversible or irreversible.One of the most well-known types of binding is the binding of proteins to other molecules. Proteins are made up of chains of amino acids and can interact with other molecules through specific binding sites on their surface. When a protein binds to another molecule, such as a ligand, it can change its shape and function in response to the binding.

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4. The traits of individuals best adapted to survive become more common in each
mew generation because

Answers

The animals with those traits have a better chance of surviving and mating.

Which of the following is most helpful for identifying an organism using a dichotomous key?
O Its average lifespan.
0 Its sources of food.
O Its habitat.
O Its physical structures.

Answers

I believe it’s by the traits, so it would be physical structures. Because of there’s only two options available, which is see better by the traits of the unknown specimens.
Lol I think thouuuu cuz I do fully remember everything.

Count then plot the total number of atoms in the reactants to total atoms in the products for the equation 2H₂ + O₂ ➜ 2H₂O
1 Reactant
2 Reactants
3 Reactants
4 Reactants
5 Reactants
6 Reactants

Count then plot the total number of atoms in the reactants to total atoms in the products for the equation

Answers

Answer:

welp its wrong i guess my bad

In 3-5 sentences, describe the advantages and disadvantages of using these two different images to model Mitosis.

In 3-5 sentences, describe the advantages and disadvantages of using these two different images to model
In 3-5 sentences, describe the advantages and disadvantages of using these two different images to model

Answers

The advantage of the first model is it used less space for showing stages of mitosis whereas the disadvantage of using this model in Mitosis is that it does not provide detailed information.  

What is mitosis cell division?

Mitosis is a process of cell division in cells that occurs when a parent cell divides to form two identical daughter cells. During this cell division, diploid daughter cells are produced from a single parent cell while on the other hand, meiosis is a process of cell division in cells that happens when a parent cell divides to form four identical daughter cells. Meiosis refers to the formation of four cells from a single parent having haploid chromosomes. The advantage of the first model is that it provides stages of mitosis by using less space whereas the disadvantage of using this model Mitosis is that it does not provide detailed information. The advantage of the second model is that it does not provide detailed information whereas the disadvantage of using this model of Mitosis is that it provides stages of mitosis by using less space.

So we can conclude that the advantage of the first model is the disadvantage of the second model and vice versa.

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Answer:

The disadvantages for both cells are that neither is an accurate representation of the cell themselves, or the processes. The first cell doesn't tell us exactly what phases the cell is currently going through, and the cell doesn't go through is phases through grey arrows either. However, it does tell us what the phases look like, and the process of how the cell goes through them. The disadvantages of the second map is that, it doesn't show what the phases look like, the advantages are that tells us what the phases are and what comes before and after them, while showing how this occurs in a cycle and keeps going until senescense.

Explanation: Please give me Brainliest! Thank you!

Explain the difference between transverse wave and longitudinal wave.


Answer :
Transverse wave is a wave which vibrates or oscillates at right angles to the direction the wave or energy is moving
Examples : light waves abd waves that are traveling on the surface of the water.

Longitudinal waves is a wave which vibrates or oscillates to the direction the energy or wave is moving
Example : sound waves​

Answers

Answer:

» A transverse wave is made up of crests and troughs at maximum displacements hence vibrating energy at right angles, where as a longitudinal wave is made up of compressions and rare factions where energy is assigned and kept in those particle density regions

Explanation:

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Why is it necessary to complete doze of vaccine

Answers

Answer:

to get longer-lasting protection

Explanation:

Answer:

HOPE THIS HELPS

Explanation:

Because Receiving only one dose is not as useful as receiving the full dose, getting both doses of these vaccines maximizes your ability to receive full immunity from the vaccine. Receiving only one dose wouldn't provide you with the full protection and does not give you the full effectiveness of the vaccine.

Which type of cells form the lining of the small intestine.

Answers

Answer:

polarized cells

Explanation:

The intestinal epithelium is lined with a single layer of polarized cells,

how is the woody xylem in tilia different than monocots and dicots

Answers

Answer:

Herbaceous stems lack woody tissue and growth rings unlike woody xylem. Wood is a composite of cellulose fibers which require the the process of phloem in the bark to contain nutrients unlike herbaceous stems that rely on xylem that contains vessel and vascular elements.

Explanation:

bennett is a 12-year-old boy whose bmi-for-age falls at the 90th percentile on the bmi-for-age growth chart. bennett is

Answers

Answer:

bennett is a 12-year-old boy whose bmi-for-age falls at the 90th percentile on the bmi-for-age growth chart. bennett is

Explanation:

bennett is a 12-year-old boy whose bmi-for-age falls at the 90th percentile on the bmi-for-age growth chart. bennett is

what types of organisms have endoskeleton​

Answers

Answer:

Endoskeletons: The skeletons of humans and horses are examples of endoskeletons. They provide bodies with support, protection of organs, and aid in movement.

Explanation:

Answer:

Mammals, reptiles, birds, fish and amphibians are vertebrates with endoskeletons.

Since seeds are plants, could the results have been influenced by photosynthesis? Why or why not and how could you tell the difference?

Answers

Photosynthesis is the process by which plants convert sunlight into energy to fuel their growth and development. While seeds contain the potential to become plants, they do not photosynthesize until they germinate and grow into seedlings with leaves.

To confirm whether photosynthesis had any effect on the study results, researchers could conduct a control experiment where they expose seeds to light but prevent photosynthesis from occurring, such as by adding a chemical that inhibits chlorophyll production.

If the results of this experiment differ from the original study, it would suggest that photosynthesis may have played a role in the initial results. However, since photosynthesis does not occur in seeds, it is unlikely to have any significant impact on a study of seed properties or characteristics.

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Which of the following descriptions matches the term near the left fifth intercostal space?
A) pericardial cavity
B) visceral pericardium
C) apex of heart
D) aorta
E) right atrium

Answers

The term "near the left fifth intercostal space" is associated with the description apex of the heart. The correct option is C.

The apex of the heart is the pointed, inferior portion of the heart, which is located near the left fifth intercostal space. The intercostal spaces are the spaces between the ribs, and the left fifth intercostal space refers to the area between the fifth and sixth ribs on the left side of the chest. The apex of the heart is where the tip of the heart is closest to the anterior chest wall.

The other options do not correspond to the specific location described. The pericardial cavity is the fluid-filled space surrounding the heart (option A). The visceral pericardium is the inner layer of the pericardium, which covers the surface of the heart (option B). The aorta is the large artery that carries oxygenated blood from the heart to the rest of the body (option D). The right atrium is one of the four chambers of the heart, located on the right side (option E).

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How does the size of a bacterial cell compare with an animal cell?

Answers

Answer:

hope it helped

Explanation:

Bacterial cells are very small - about 10 times smaller than most plant and animal cells. Most bacterial cells range in size from 0.2 to 10 microns or micrometers (0.0000079 to 0.00039 inches). ... One reason why bacterial cells are so small is that they need a large surface area to cell volume to take in nutrients.

A mutation happens in the ______.​

A mutation happens in the ______.

Answers

DNA !! Can I have brainlyist please :))

Using what you know about plate boundaries, explain the tectonic movement that is occurring at the East African Rift Valley, and predict how the area may change in the future.

Answers

Answer: The East African Rift Valley (EAR) is a developing divergent plate boundary in East Africa. ... The Nubian and Somalian plates are also separating from the Arabian plate in the north, thus creating a 'Y' shaped rifting system. These plates intersect in the Afar region of Ethiopia at what is known as a 'triple junction'.  Hope this helps! <3

The tectonic movement that is occurring in the East African Rift Valley gradually develops plate boundaries within the region of East Africa.

What is the Tectonic movement?

The tectonic movement may be defined as the migration of the plates that construct up the surface of the Earth's crust. The Earth is made up of roughly a dozen major plates and several minor plates. These plates are moved due to some extension of pressure applied on them.

Due to this gradual movement of plate tectonics, the area of the region may greatly change from physical as well as climatic perspectives. This is due to the Convection currents that drive the movement of Earth's rigid tectonic plates in the planet's fluid molten mantle.

Therefore, the tectonic movement that is occurring in the East African Rift Valley gradually develops plate boundaries within the region of East Africa.

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A Stratigraphic column is a cross section of rock, shows a sequence of sedimentary rocks, with the oldest rocks on the bottom and the youngest on top

-True
-False

Answers

A Stratigraphic column is a cross section of rock, shows a sequence of sedimentary rocks, with the oldest rocks on the bottom and the youngest on top. This is True.

What is a stratigraphic column?

A stratigraphic column is a visual representation of a sequence of sedimentary rocks arranged in chronological order, with the oldest rocks located at the bottom and the youngest rocks at the top. The column is typically created by studying the layers of rocks found in a particular area, and it provides a record of the geological history of that area.

A sedimentary rock is a type of rock that forms from the accumulation and cementation of sediment particles, such as sand, mud, clay, and other fragments of rock, shells, or fossils.

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Give the complementary DNA sequence from the following mRNA sequence UGGCUAAOCG

Answers

Answer:

ACCGAUU GC

Explanation:

not sure what the o is supposed to be but if its a c then a g goes in the space l left

if a person does not eat enough carbohydrate to supply the glucose the body needs

Answers

Carbohydrates are a vital part of the human body's energy source. The body produces glucose when it digests carbs, and it uses glucose to power the muscles and organs. It's essential to consume enough carbohydrates to meet the body's energy demands, but if a person doesn't eat enough carbohydrate to supply the glucose the body needs, it can cause some health problems.

When a person doesn't eat enough carbohydrates, the body has to break down proteins and fats to generate energy. When proteins and fats are used for energy, the body produces waste products like ketones. High levels of ketones can lead to a condition known as ketosis, which can cause a range of unpleasant symptoms.

In addition to ketosis, not eating enough carbs can also cause low blood sugar, also known as hypoglycemia. Hypoglycemia occurs when there isn't enough glucose in the bloodstream to provide the body's energy needs. Symptoms of hypoglycemia include shaking, sweating, confusion, and fatigue.

Moreover, not consuming enough carbs can lead to other health problems like headaches, constipation, and nutrient deficiencies. Carbohydrates provide an excellent source of fiber, vitamins, and minerals that the body needs to function correctly.

In conclusion, it's essential to consume enough carbohydrates to supply the glucose the body needs. If a person doesn't eat enough carbs, the body will have to break down proteins and fats to produce energy, which can lead to ketosis and hypoglycemia, as well as other health problems.

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What is the most important event during S-phase?

Answers

Answer:

The most important event occurring in S phase is the replication of DNA. The aim of this process is to produce double the amount of DNA, providing the basis for the chromosome sets of the daughter cells.

Explanation:

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Have A Great Day

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The photos below show specialized cells of a plant.
In which specialized organ of the plant would these cells most likely be
found?
O
A. A stem
OB. A leaf
O C. A flower
OD. A root

The photos below show specialized cells of a plant.In which specialized organ of the plant would these

Answers

Answer:

B. Leaf

Explanation:

It looks to be green, which is the color of chlorophyll produced in the chloroplasts. This means that it is found in the green leaves and is used to convert light into energy.

in order to maintain their organization and carry out metabolic activities, organisms need a constant source of

Answers

In order to maintain their organization and carry out metabolic activities, organisms need a constant source of energy, as energy is needed to carry out a variety of processes, such as growth, repair, movement, and reproduction.

What is the importance of energy in organisms?

 

Energy is needed for metabolic reactions, for walking, for doing daily activities, and the energy they can get from different sources such as from plants, from cellular respiration, etc.

Hence, in order to maintain their organization and carry out metabolic activities, organisms need a constant source of energy, as energy is needed to carry out a variety of processes, such as growth, repair, movement, and reproduction.

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How can rocks be turned into sediment?

Answers

Weathering/Erosion breaks down the rock into sand, pebbles and mud to create sediment.

Which is an example of genetic diversity? PLS HELP temperate rain forest and a tropical rain forest bees, jack rabbits, diamondback rattlesnakes bacteria, garter snakes, tigers variations in a flock of starlings

Answers

Answer:

temperate rain forest and a tropical rain forest bees.

Explanation:

The temperate rain forest and a tropical rain forest bees is the example of genetic diversity because the same organisms has different features which allows them to live in different climatic conditions due to their variation in genetics. The environment of temperate rain forest is different from tropical rain forest so the bees specie that live in both environment are different from one another in their genetic makeup and which is an example of genetic diversity.

Which organelle controls everything in the cell?
Mitochondria
Cytoplasm
Nucleus
Ribosomes

Answers

The nucleus controls everything in the cell

Answer:

Nucleus

Explanation:

The nucleus controls all of the cell's activities, such as growth and metabolism, using the DNA's genetic information.

sterols are precursors for vitamin d and cholesterol true or false

Answers

Sterols are precursors for vitamin D and cholesterol is a true statement. Sterols are a kind of fat found in plants and animals that have a steroid-like chemical structure and have been linked to human health benefits.

They are also known as steroid alcohols, and they are an essential part of the body's cell membranes. A precursor is a substance that the body converts into another chemical or compound. Precursors are intermediaries in the body's metabolic processes, converting one substance into another.

The statement Sterols are precursors for vitamin D and cholesterol is true.  7-dehydrocholesterol, which is derived from cholesterol, is a precursor to vitamin D3, which is then converted into calcitriol, the active form of vitamin D that is used by the body.

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Steady callin' my phone
I done told you before that it's over, leave me 'lone
Know it's hurtin' you to see me gone
Dark clouds, you gon' see me storm
I won't go back
But trust me, you're gon' hold that
Hold that

Answers

Answer:

that some nice

so nice i made a remix

Steady callin' my ex

I done told you before that im g.ay,leave me be

Know your actually happy im gone

Dark clouds, you gon' see me fart aggressively

I won't hold back

But trust me, you're gon' smell that

smell that

Explanation:

List the following objects from largest to smallest

Universe, planets, sun, asteroids, galaxy, meteors

Answers

Universe galaxy sun planets asteroids meteors
From largest to smallest they are: Universe, galaxy, solar system, star, planet, moon and asteroid

Why are indicated gene expression in stem cells versus specialized ( differentiated) cells

Answers

Gene expression in stem cells is more versatile and pluripotent, allowing them to differentiate into various cell types, while specialized cells have specific gene expression patterns that enable them to perform their specialized functions

Gene expression in stem cells and specialized (differentiated) cells differs due to their distinct roles and functions in the body. Stem cells have the unique ability to differentiate into various cell types, whereas specialized cells have already undergone differentiation and acquired specific functions.

In stem cells, gene expression is more pluripotent, meaning a wide range of genes can be activated or repressed to maintain their self-renewal and differentiation potential. This flexibility allows stem cells to respond to different signals and stimuli, enabling them to give rise to different cell lineages during development or tissue regeneration.

On the other hand, specialized cells have undergone a process called cell differentiation, where specific genes are selectively expressed or silenced to establish their specialized functions and morphology. Gene expression in specialized cells is more restricted and specific, as it is essential for the cell to perform its designated role effectively and maintain tissue homeostasis.

The regulation of gene expression in stem cells and specialized cells involves complex molecular mechanisms, including the activation or repression of transcription factors, epigenetic modifications, and signaling pathways. These mechanisms ensure the appropriate gene expression patterns in different cell types, allowing for proper development, tissue function, and overall organismal health.

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When do the first land vertebrates appear in the geologic time scale?

Answers

Answer:

About 450 million years ago

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