in an sds-page experiment proteins are separated by a) negatively charged sidechains. b) charge-to-mass ratio. c) molecular weight. d) positively charged sidechains. e) different isoelectric points.

Answers

Answer 1

In an SDS-PAGE experiment, proteins are separated by their molecular weight.  The correct option is c).

The SDS detergent coats the proteins with a negative charge and denatures them, causing them to unfold into linear chains. As the proteins migrate through the gel, they are separated based on their size, with smaller proteins moving faster and larger proteins moving slower.

This separation is due to the charge-to-mass ratio of the proteins, with the negatively charged SDS molecules interacting with the positively charged amino acid sidechains in the proteins.

The separation is not based on the isoelectric point of the proteins, as all proteins have been denatured and coated with SDS, eliminating any charge differences based on the isoelectric point.

In an SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) experiment, proteins are separated based on their molecular weight. SDS is a detergent that denatures the proteins and gives them a uniform negative charge, which cancels out any inherent charge-to-mass ratio or isoelectric point.

During electrophoresis, the proteins are placed in a gel matrix and subjected to an electric field. The negatively charged proteins move towards the positively charged electrode, with smaller proteins moving faster through the gel than larger proteins.

As a result, the proteins become separated based on their molecular weight, with smaller proteins migrating farther down the gel than larger proteins.

The gel can be stained to visualize the separated proteins, which can be used to analyze the protein composition of a sample or to confirm the expression and purification of a particular protein of interest.

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Related Questions

What do the results tell us about the experiment? The bacteria from all 3 farms were almost 100% alive in the control samples, meaning that the experiment did not work. None of the bacteria from the 3 farms survived in the control samples. Because the bacteria in the control were almost 100% alive, we know that any deaths of bacteria in the other samples was because of the antibiotics.

Answers

Answer: D

Explanation: The ones who are doing this on Gizmos the answer is D.

“Because the bacteria in the control were almost 100% alive, we know that any deaths of bacteria in the other samples was because of the antibiotics.” Y’all should like my answer n give me the brain answer.

The results tell us that in the experiment the death of the bacteria in the other samples was because of the antibiotics, confirming that the bacteria from the farms are sensitive to antibiotics.

What does it mean that the antibiotic is sensitive to bacteria?

If a bacterium undergoes the action of an antibiotic, it is said to be sensitive; if, on the contrary, the antibiotic has no effect on the bacterium, it is said to be resistant. Resistance can be natural or acquired (eg, by plasmid transfer or mutations).

With this information, we can conclude that the results tell us that in the experiment the death of the bacteria in the other samples was because of the antibiotics, confirming that the bacteria from the farms are sensitive to antibiotics.

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What are two areas in the cerebral hemispheres responsible for speech? in which lobes of the brain are they located?.

Answers

Wernicke's area and Broca's area in the cerebral hemispheres responsible for speech.

Broca's and Wernicke's areas are cortical areas that specialize in human language production and comprehension, respectively. Wernicke area, a region of the brain containing motor neurons involved in speech comprehension. Carl Wernicke, a German neurologist, described this area for the first time in 1874. The Wernicke area is located in the posterior third of the left hemisphere's upper temporal convolution.

Broca's area is an important component of a complex speech network, interacting with the flow of sensory information from the temporal cortex, developing a plan for speaking, and passing that plan on to the motor cortex, which controls mouth movements.

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the _____ is critical for object recognition, whereas the _____ is critical for vision for action.

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Answer:

the __ventral stream __ is critical for object recognition, whereas the __dorsal stream___ is critical for vision for action.

paba serves as the competitive inhibitor in the action of sulfanilamides. group of answer choices true false

Answers

True. PABA (para-aminobenzoic acid) serves as a competitive inhibitor in the action of sulfanilamides.

Sulfanilamides are a class of antibiotics that work by inhibiting the growth of bacteria. They do so by blocking the synthesis of folic acid, which is essential for the bacteria's survival. PABA is a precursor in the synthesis of folic acid, and sulfanilamides structurally resemble PABA.

By acting as competitive inhibitors, sulfanilamides compete with PABA for the active site of the enzyme involved in folic acid synthesis. This competition prevents PABA from binding to the enzyme and thus disrupts the production of folic acid, ultimately inhibiting bacterial growth.

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what is the connection between poverty and food deserts?.(ecology)

Answers

Answer:

it is a connection problem

both of them are problems

9. PLEASE HELP A LOT OF POINTS

9. PLEASE HELP A LOT OF POINTS

Answers

Answer:

im pretty sure its D

Explanation:

sorry if im wrong

Ice is less dense than water, which allows it to float on liquid water. Why does this happen?
A. Because the ice molecules are spaced closer together
B. Because the ice molecules are spaced further apart
C. Because the liquid water molecules are arranged in the shape of a pentagon
D. Because the liquid water molecules are arranged against their polarity

Answers

Answer:

D

Explanation:

The ice molecules are closer together, so it is more rigid.

Ice is less dense than water, which allows it to float on liquid water because the liquid water molecules are arranged against their polarity. Thus option D is correct.

What are the properties of water ?

water is in liquid state, hydrides of the oxygen family bound by hydrogen bonding, colorless, odorless, tasteless liquid, hexagonal crystal structure.

The density of water is about 1 gm/cc and varies with changing temperature, viscosity of water is 0.89 cP, boiling point is 100°C, freezing point changes from liquid to solid which is about 0°C or 32°F.

The hydrogen bonds form water freezes into ice allow the water molecules to be spaced farther apart, which making them to take more space, decrease the overall density and making water to float than ice.

Thus option D is correct.

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Which scenario causes gene flow

Answers

Answer: Migration is one way that genes are transferred from one population to another.

Explanation:  Gene flow is caused by a significant change in the population of individuals due to the addition of other individuals whose alleles are slightly different from those of the existing individuals

Answer:

Introgression refers to gene flow from another taxon. While low levels of gene flow between species are not uncommon, it is a serious issue when an introduced invasive species hybridizes with a native species and overwhelms its genetic constitution (genetic swamping). This is a major threat in canids, ducks, fish and plants. For example, exotic rainbow trout introduced into the habitats of native cutthroat trout in the Western United States led to hybrid swarms that eventually overwhelmed the local species. Introgression is readily detected by molecular genetic and genomic methods.

Explanation:

HOPE ITS USEFUL

Name two major types of mutations. What do they have in common? How are theydifferent? Give an example of each.

Answers

Answer:

Two major categories of mutations are germline mutations and somatic mutations.

Germline mutations occur in gametes. These mutations are especially significant because they can be transmitted to offspring and every cell in the offspring will have the mutation.

Somatic mutations occur in other cells of the body.

Two major categories of mutations are germline mutations and somatic mutations.
Germline mutations occur in gametes. These mutations are especially significant because they can be transmitted to offspring and every cell in the offspring will have the mutation.
Somatic mutations occur in other cells of the body.

Classify) While walking in a park, Mia observes grasses, shrubs, wildflowers, rocks, algae, a stream, water plants, frogs, turtles, birds, a squirrel, and a snake. What is one way Mia can organize these by level of organization?

Answers

Mia can organize the observed elements by the level of the organization using an ecological framework.

At the lowest level, there are individual organisms such as grasses, shrubs, wildflowers, rocks, algae, frogs, turtles, birds, a squirrel, and a snake. These represent the specific living entities. Moving up, these organisms form populations, including populations of frogs, turtles, birds, and so on. Populations interact to create a community, which encompasses grasses, shrubs, wildflowers, rocks, algae, frogs, turtles, birds, a squirrel, and a snake.

Finally, these communities, along with the abiotic factors like the stream and water plants, form an ecosystem. This hierarchical organization captures the relationships and interdependencies among the observed elements in the park.

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60 milligrams=____kg?

4.5 centimeters=___km?

Answers

Answer:

60 milligrams= 6e-5 kg?

4.5 centimeters= 4.5e-5 km?

Explanation:

Why do smokers find it harder to give up smoking? ​

Answers

Answer:

Nicotine is the main addictive drug in tobacco that makes quitting so hard

Answer:

It is hard for them because that's what makes them feel better and that is what the want each time they are stressed or they can even smoke 20 times in a hour.

Explanation:

Hope i helped :)

Achondropladia, a form of dwarfism, is inherited as a dominant condition (A). What is the probability of two heterozygous dwarfs having normal height children? Show your work.

Probability:

Achondropladia, a form of dwarfism, is inherited as a dominant condition (A). What is the probability

Answers

In this scenario, the genotype of the two heterozygous dwarfs can be represented as Aa x Aa, where A represents the dominant allele for achondroplasia and a represents the recessive allele for normal height.

The Punnett square for this cross would be:

( picture in the attachment )

As shown in the Punnett square, there is a 25% chance that a child would inherit two recessive alleles (aa) and have normal height, while there is a 75% chance that a child would inherit at least one dominant allele (Aa or AA) and have achondroplasia.

Therefore, the probability of two heterozygous dwarfs having normal height children is 25%, or 1/4.

It is important to note that this is a theoretical probability based on Mendelian genetics, and that actual inheritance patterns may vary due to factors such as incomplete dominance or gene interactions.

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Achondropladia, a form of dwarfism, is inherited as a dominant condition (A). What is the probability

Which best describes the scientists who contribute to our current body of knowledge?

Answers

Scientists are driven by curiosity, possess critical thinking skills, and are dedicated to the pursuit of knowledge. They contribute to our current body of knowledge through meticulous research, collaboration, and a commitment to evidence-based reasoning, ultimately advancing our understanding of the natural world and shaping the progress of scientific discoveries.

Scientists who contribute to our current body of knowledge are individuals dedicated to the pursuit of understanding and expanding our understanding of the natural world. They are driven by curiosity, the desire to explore unanswered questions, and the passion for discovery.

These scientists possess several key characteristics. Firstly, they exhibit a strong scientific mindset, which includes critical thinking skills, skepticism, and a commitment to evidence-based reasoning. They are meticulous in their research methodologies, conducting experiments, gathering data, and analyzing results with precision and rigor.

Additionally, scientists are often highly knowledgeable in their respective fields, having obtained advanced degrees and specialized training. They stay up-to-date with the latest research and developments in their areas of expertise, constantly expanding their understanding and building upon existing knowledge.

Scientists are also collaborative and actively engage in scientific communities. They share their findings through scientific publications, presentations at conferences, and discussions with their peers. They seek feedback, engage in constructive debates, and participate in interdisciplinary collaborations to tackle complex problems.

Moreover, scientists are resilient and persistent. They encounter setbacks, face challenges, and encounter failures along their scientific journey. However, they view these obstacles as opportunities for growth and learning, adapting their approaches and methodologies to overcome obstacles and advance their understanding.

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why do the potato strip in pure water get bigger?​

Answers

Answer:

The incoming water in the potato cells pushes on the cell walls and makes the cells bigger , that's how the strips of potato in pure water becomes bigger .

This is because of osmosis, water from outside the potato strip travels down the concentration gradient and into the potato strip causing it to get bigger.

What is the role of lysosomes in animal cells?

Answers

lysosomes contain digestive enzymes which help break down excess or worn-out cell parts

Which organism commonly causes food poisoning by consumption of foods containing excessive populations of organisms or preformed enterotoxin?

Answers

According to the given statement Bacillus cereus It is the cause of two different kinds of food poisoning.

What does the term "etiological agents" mean?

Etiologic agents include those mentioned in 42 CFR 72.3 as well as any other agent that causes or may cause a severe, incapacitating, or deadly disease. Etiologic agents are living microorganisms or their toxins.

How many different etiological agent categories are there?

The five classes of pathogenic organisms include viruses, bacteria, fungus, protozoa, and helminths (worms).

What are the modes of transmission for the etiologic agent?

Direct touch, droplets, a vector like a mosquito, a vehicle like food, or the airborne route are all possible modes of transmission. The vulnerable host has several portals of entry such as the mouth or a syringe.

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Question 7 For the 14.317 g sample of sodium sulfate, how many oxygen atoms do you have?
Do not include units. Make sure to pay attention to how many significant figures your answer should have.
Note: Your answer is assumed to be reduced to the highest power possible.
Your Answer:
x10
Answer

Answers

Mass of sodium sulfate = 14.317 g

To find:

Number of oxygen atoms in the given mass of sodium sulfate (Na₂SO₄)Let's first find the formula mass of sodium sulfate (Na₂SO₄)Molecular mass of Na = 23 g/molMolecular mass of S = 32 g/molMolecular mass of O = 16 g/mol Atomic mass of Na₂SO₄ = 2 × 23 + 1 × 32 + 4 × 16 = 46 + 32 + 64 = 142 g/mol Number of moles of sodium sulfate = Given mass / Atomic mass = 14.317 / 142 = 0.1007 molesWe know that in one mole of sodium sulfate (Na₂SO₄), there are 4 oxygen atoms. Therefore, in 0.1007 moles of sodium sulfate,Number of oxygen atoms = 0.1007 × 4 = 0.4028 ≈ 0.403 ≈ 4.03 × 10¹ = 4.03 x 10¹So, the number of oxygen atoms in the given mass of sodium sulfate (Na₂SO₄) is 4.03 × 10¹.

About Sodium sulfate

Sodium sulfate is an inorganic compound with the chemical formula Na₂SO₄ with some of its hydrates. All sodium sulfates are white solids which are very soluble in water. Its annual production reaches 6 million tons per year, with decahydrate as its main commodity product.

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If you have 50 mg of starch, how much water needs to be added to make a concentration of 5g/mL.

Answers

1:5 dilution = 1/5 dilution = 1 part sample and 4 parts diluent in a total of 5 parts. If you need 10 ml, final volume, then you need 1/5 of 10 ml = 2 ml sample. To bring this 2 ml sample up to a total volume of 10 ml, you must add 10 ml - 2 ml = 8 ml diluent.

an antifungal combination matrix identifies a rich pool of adjuvant molecules that enhance drug activity against diverse fungal pathogens

Answers

The study involving antifungal combination matrix identifies a rich pool of adjuvant molecules that enhance drug activity against diverse fungal pathogens.

A matrix of drug combinations has been used to identify antifungal adjuvant molecules. The aim of the study was to discover molecules that enhance the activity of antifungal drugs against Candida albicans, which causes candidiasis, and Aspergillus fumigatus, which causes aspergillosis.

They discovered 10 molecules that improved the activity of an antifungal drug against C. albicans, as well as four molecules that improved the activity of an antifungal drug against A. fumigatus.Antifungal combination matrix is a process in which various antifungal agents are combined to identify the best combination of agents that can treat or prevent fungal infections.

Antifungal drugs are used to treat fungal infections that can affect various parts of the body, such as the skin, lungs, and nails. Antifungal combination therapy can help to prevent the emergence of drug-resistant fungi by preventing the fungi from developing resistance to a single antifungal agent.

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True or False: Every mature MHC-restricted T cell expresses both CD4+ and CD8+ co-receptors.

Answers

The given statement "Every mature MHC-restricted T cell expresses both CD4+ and CD8+ co-receptors." is false because when T-Cell matures and leaves the thymus, expresses only one type of co-receptor.

Mature MHC-restricted T cells do not express both CD4+ and CD8+ co-receptors. The expression of CD4 or CD8 co-receptor is mutually exclusive and depends on the functional subtype of the T cell. CD4+ T cells, known as helper T cells, express the CD4 co-receptor and recognize antigens presented by MHC class II molecules on antigen-presenting cells.

They play a crucial role in coordinating immune responses. On the other hand, CD8+ T cells, known as cytotoxic T cells, express the CD8 co-receptor and recognize antigens presented by MHC class I molecules on infected or abnormal cells.

These cells are responsible for directly killing target cells. The expression of CD4 or CD8 co-receptor determines the functional specialization of T cells, and therefore, each mature MHC-restricted T cell expresses either CD4 or CD8, but not both co-receptors.


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Galapagos Finches living on different islands feed on a variety of food sources, depending on what is available from one island to the next. This has resulted in what adaptation?

Answers

Answer:

behavioral adaptation

Explanation:

behavioral adaptation is an adaptation that is not structural or physiological

Use one of the properties of water to explain why, on a very hot and sunny day, outdoor swimming pools are able to stay cool.

Answers

Answer:

what are we supost to do

Explanation:

Answer:

This is due to evaporation. When you get out of the swimming pool, the water is evaporating from your skin. This process is takes a lot of energy, and it takes that energy in the form of heat from your body.

Imagine you changed the concentration of K outside a neuron such that the resting membrane potential changed to -80 mV (from the normal resting value of -70 mV). What have you changed

Answers

When the concentration of K outside a neuron changes, it affects the resting membrane potential. The resting membrane potential is the electrical charge across the cell membrane when the neuron is at rest. This potential is maintained by the uneven distribution of ions across the membrane, with more K ions inside the cell and more Na ions outside the cell.

When the concentration of K outside the neuron changes, it alters the electrochemical gradient, causing K to diffuse out of the cell. This leads to hyperpolarization of the membrane potential, meaning it becomes more negative than the normal resting value of -70 mV.
Therefore, if the resting membrane potential changes to -80 mV, it indicates that the concentration of K outside the neuron has increased. This change in ion concentration alters the balance of ions across the membrane, and the neuron may be less excitable, which can impact its ability to transmit signals.
Overall, altering the concentration of K outside a neuron can affect the resting membrane potential, which in turn influences the neuron's ability to transmit signals and carry out its functions.

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What is the cytoplasm in a slug's cells called?

Answers

Answer:

The main components of the cytoplasm are cytosol (a gel-like substance), the organelles (the cell's internal sub-structures), and various cytoplasmic inclusions. The cytoplasm is about 80% water and usually colorless.

Answer:

the plazma membrain

Explanation:

An organisms that produce its own food source using the energy form the sun or from breaking down inorganic molecules is termed a(n)

Answers

An organism that produces its own food source using the energy from the sun or from breaking down inorganic molecules is termed an autotroph. Autotrophs are able to convert energy from sunlight or inorganic compounds into organic molecules through the process of photosynthesis or chemosynthesis, respectively.

In photosynthesis, autotrophs such as plants, algae, and some bacteria use sunlight to convert carbon dioxide and water into glucose and oxygen. This process occurs in the chloroplasts of plant cells.

In chemosynthesis, certain bacteria and archaea can obtain energy by breaking down inorganic compounds like hydrogen sulfide, ammonia, or iron, and use that energy to synthesize organic molecules.

Autotrophs play a crucial role in ecosystems as they are the primary producers, forming the basis of the food chain by providing energy and nutrients for heterotrophs, which are organisms that cannot produce their own food and rely on consuming other organisms.

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Does DNA contains all the genetic information within an organism ? True or false

Answers

Answer:

DNA does contain all genetic information within an organism I believe?

Answer:

True

Explanation:

An organism's complete set of nuclear DNA is called its genome. Besides the DNA located in the nucleus, humans and other complex organisms also have a small amount of DNA in cell structures known as mitochondria. Mitochondria generate the energy the cell needs to function properly.

in muscles during strenuous exercise, under anaerobic conditions lactic acid builds up due to the following reaction. The carbon atom indicated by the asterisk is (a) chiral (b) prochiral (c) achiral (d) both achiral and prochiral

Answers

The carbon atom indicated by the asterisk is prochiral and achiral. so, option (b) and (c) is correct.

What is reaction ?

A chemical reaction is the transformation of one or more chemicals, known as reactants, into one or more new compounds, known as products. The change in concentration of any of the reactants or products per unit of time can be used to determine the rate or speed of a reaction. It is determined by the equation rate=time + concentration.

Exercise is physical activity that is organized, prescribed, and repeated with the goal of conditioning any area of the body. Exercise is crucial for physical rehabilitation as well as for maintaining fitness and enhancing health that helps our body in metabolic reactions.

Therefore, carbon atom indicated by the asterisk is prochiral and achiral. so, option (b) and (c) is correct.

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Which of the following Venn diagrams would BEST be suited to represent the three categories of animals?

Which of the following Venn diagrams would BEST be suited to represent the three categories of animals?

Answers

Answer:

intersecting circle and the universal affarmative

Explanation:

If an F1 plant, which is the progeny of RR and rr plants, is backcrossed to the parent with round seeds, what proportion of the progeny will have wrinkled seeds? (Use a Punnett square.)

Answers

To answer this question, we need to understand the genetics of seed shape in plants. The shape of the seed in pea plants is controlled by a single gene, with two possible alleles: R for round seeds and r for wrinkled seeds. When a plant with two copies of the same allele (RR or rr) is crossed with a plant with two copies of the other allele (rr or RR), the offspring will be heterozygous (Rr) and have round seeds because the dominant R allele masks the recessive r allele.

Now, if we take an F1 plant that is heterozygous (Rr) and backcross it to the parent with round seeds (RR), we can use a Punnett square to predict the possible genotypes and phenotypes of the offspring.

The Punnett square would look like this:

      R   R
   Rr  RR  Rr
   Rr  RR  Rr

So, out of four possible offspring, two will be RR and two will be Rr. This means that 50% of the progeny will have wrinkled seeds (rr genotype) and 50% will have round seeds (Rr genotype).

In conclusion, when an F1 plant, which is the progeny of RR and rr plants, is backcrossed to the parent with round seeds, 50% of the progeny will have wrinkled seeds.

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Martha, a first-grade teacher, is worried that if doesn't stay In at work to grade papers her principal will think she is lazy She is also worried that if she stays late at work her partner will be angry. This of conflict is known as In the philosophical area of metaphysics there is a subfield called ontology, which focuses on figuring out how to define & distinguish between kinds of being. According to reading 8 ( introduction to metaphysics) , there is a major division between material & immaterial things . Give an easy/obvious example for both categories. Now , find something hard to categorize & explain why you think its difficult.Now, recall that some theories of reality consider certain kinds of beings more fundamentally real than other kinds of beings. Looking at three examples you just gave; which being would Epicurus consider more real than the others & why ? when critical thinkers gather information and ideas, the first step they take is to multiple choice make a list of what information is needed and where it can be found. decide on methods for gathering information. identify holes and weaknesses. collect needed resources by assigning members specific responsibilities for items in the master list. take stock of existing information. Differential AnalysisIn each of four independent cases, the amount of differential revenue or differential cost is as follows (parentheses indicate decreases):1234Increases (decreases) in:Revenue$14,000$0??Costs??$(16,000)$0For each case, determine the missing amount that would be necessary for the net differential amount to be:a. $10,000Use a negative signs with answers to reflect decreases.1234Increases (decreases) in:Revenue$14,000$0AnswerAnswerCostsAnswerAnswer$(16,000)$0b. ($2,000)Use a negative signs with answers to reflect decreases.1234Increases (decreases) in:Revenue$14,000$0AnswerAnswerCostsAnswerAnswer$(16,000)$0 WILL GIVE BRAINLIEST!If a + b = c and a = 4, b = 12 then find c.Round your answer to the nearest hundredth.Question 4 options:A. c = 12.6B. c = 12.64C. c = 11.31D. c = 12.65 How might the growth of trade help the rise of renaissance in Italy The large number before a molecule is called what What is the purpose of Green building? a. To get federal funding c. To recycle building materials b. To create a harmonious relationship with the environment d. To lower energy bills If we have too many passenger at a bus or train station, what may happen? For each repetition of a binomial experiment, what are the two mutually exclusive outcomes?. The graph shows the size of two populations of the same species over time.35302520Number of Individuals15-Area AArea B501 2 3 4 5 6 7 8 9 10 11 12 13 14 15TimeWhich of these limiting factors is likely to be more plentiful in area A than inarea B? Which of the following would weather forecasters be concerned about predicting in the event that certain geographic area received an excessive amount of rain in a short period of time?A - FloodingB - TyphoonsC - Hurricanes D - tidal Waves Which of the following is the number of moles of a solute that is dissolved in 1 liter of solution? A. mass percent B. molality C. molarity D. mole fraction A car drives 48 m/s for 5 minutes. What distance does the car travel? Which of the following was a motivator for McCarthyism? Please Help!! For each equation, find when y when x = 03. Then, find when x when y = 2. I need help fast! John bought some Nikes at Foot Locker. They were $289.89 and he got them on sale for 20% off.1. What was the amount of the discount?2. What did he pay for the Nikes?Sam bought the same pair at another chain. His were $299.99 and 25% off.3. What was Sam's discount/savings on his shoes?4. What did he pay after the savings?5. Who got the better deal? And by how much? Write the explicit formula for the given sequence.1.5, 2.25, 3, 3.75, ...need done asap Write the correct form of the verb to complete the sentence.We (deliver) _________________ your order early next week. You have a metal with mass 15.32 g and a volumetric flask with water with mass 115.16 g. The mass of the volumetric flask with water and the metal is now 128.77 g. The empty volumetric flask had a mass of 65.03 g. What is the mass of the water displaced by the metal in grams? Report your answer to two decimal places and do not include units in your answer.