monosaccharide profiling of glycoproteins by capillary electrophoresis with contactless conductivity detection
Monosaccharide profiling of glycoproteins by capillary electrophoresis with contactless conductivity detection is saccharides.
What are saccharides?Saccharides form one of the major constituents of the biological macromolecules in living organisms. Many biological processes including the protein folding, stability, immune response and receptor activation are regulated by glycosylation.
In this work, we optimized a capillary electrophoresis method with the capacitively coupled contactless conductivity detection for separation of eight monosaccharides commonly found in glycoproteins, namely D-glucose, D-galactose, D-mannose, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, D-fucose, N-acetylneuraminic acid, and D-xylose. A highly alkaline solution of 50 mM sodium hydroxide, 22.5 mM disodium phosphate, and 0.2 mM CTAB (pH 12.4) was used as background electrolyte in a 10 µm id capillary. To achieve baseline separation of all the analytes, a counter-directional pressure of –270 kPa was applied during the separation. The limits of the detection of our method were below 7 µg/ml (i.e., 1.5 pg or 1 mg/g protein) and the limits of quantification were below 22 µg/ml (i.e., 5 pg or 3 mg/g protein). As a proof of the concept of our methodology, we performed an analysis of monosaccharides released from fetuin glycoprotein by acid hydrolysis. The results show that, when combined with an appropriate pre-concentration technique, developed method can be used as a monosaccharide profiling tool in glyco-proteomics and complement the routinely used LC-MS/MS analysis.
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what is a gene? what is a gene? a portion of rna that contains information to produce a protein. a portion of dna that contains information to produce a protein. a portion of dna that contains information to produce additional dna. a portion of a protein that contains information to produce dna.
The fundamental physical and physiological unit of heredity is a gene. DNA constitutes genes. Some genes serve as instructions for producing molecules known as proteins.
Many genes, however, don't really code for proteins. Messenger RNA (mRNA) is the name given to the type of RNA that conveys the genetic information from Dna out of the nucleus and into the cytoplasm to create a protein. In order to convert a gene into a protein, translation must take place in the cytoplasm. genetic material that is transferred from generation to generation. The chromosomes in the nucleolus have precise sites where genes are ordered sequentially on DNA sequences.
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You want to study the relationship between daily alcohol consumption and prostate-specific antigen levels. It was suggested that these two parameters have a linear relationship and as you increase your alcohol consumption your prostate-specific antigen level increases. What kind of a linear model would represent this relationship?
The kind of linear model that would represent this relationship is a positive linear model.
In a positive linear model, as the independent variable (alcohol consumption) increases, the dependent variable (prostate-specific antigen levels) also increases in a linear fashion. This implies that there is a positive correlation between the two variables, suggesting that higher alcohol consumption is associated with higher levels of prostate-specific antigen.
A linear model represents a straight-line relationship between the variables, and in this case, it would show a positive slope. The slope of the line indicates the rate at which the prostate-specific antigen levels increase with each unit increase in alcohol consumption.
By studying this relationship, researchers can gain insights into the impact of alcohol consumption on prostate-specific antigen levels and potentially understand the association between alcohol and prostate health.
However, it is important to note that further research and analysis are necessary to establish a causal relationship and consider other factors that may influence prostate-specific antigen levels.
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what is cell specialization in a multicellular organism
Answer:
the process by which generic cells change into specific cells meant to do certain tasks within the body.
Explanation:
hope this helps
Define polar molecule or polarity.This is not a test question, could you please simplify the response to as few sentences as possible?
Polarity is caused by electronegativity. Electronegativity is the ability of an atom to attract electrons and when in a molecule there is a region of low electronegativity and a region of high electronegativity we say that the molecule is polar.
When temperature in the environment drops suddenly, a bacterial cell can counteract the drop in temperature by changing its membrane lipid composition in the following way:
A. Having more long chained cis lipids
B. Having more short chained cis lipids
C. Reducing cholesterol
D. Having more saturated fatty acids
E. Both B and C
When temperature in the environment drops suddenly, a bacterial cell can counteract the drop in temperature by changing its membrane lipid composition in having more short-chained cis lipids and reducing cholesterol, option E is correct.
It does so by increasing the proportion of short-chained cis lipids and reducing cholesterol content. Short-chained cis lipids have a lower melting point, making the membrane more fluid and flexible at lower temperatures. By incorporating more of these lipids, the cell can maintain proper membrane fluidity, allowing essential cellular processes to continue.
Reducing cholesterol content is also beneficial because cholesterol tends to make the membrane more rigid. By decreasing cholesterol levels, the cell can enhance membrane fluidity and adapt to the colder environment more effectively, option E is correct.
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Which i an abiotic factor?
O A) fungi and mo on a rotting log
O B) a foret of deciduou tree
O C) a polar bear on an ice floe
O D) an ocean current of cold water
An ocean current of cold water is the abiotic factor. Abiotic factor is define as the non-living components.
Abiotic factors are non-living components of an ecosystem that influence their surroundings. Examples could be light, water, and temperature in a terrestrial habitat. Abiotic elements in a marine ecosystem include salinity and ocean current.
The living species that directly or indirectly affect other organisms in an environment are referred to as biotic components. For instance, consider plants, animals, bacteria, and the waste products they produce. The non-living, or abiotic, aspects of an ecosystem include all chemical and physical substances.
So, the fungi mould on the rotting log, a forest of deciduous tree, a polar bear on an ice floe are biotic factors. and An ocean current of cold water is abiotic factor.
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Imagine that you collected a sample of fish from a pond. Here is what you caught:
bluegill = 12 individuals
bass = 7 individuals
catfish = 6 individuals
carp = 13 individuals
What would the Shannon diversity value be for this sample?
The Shannon diversity value for the fish sample is approximately 1.791, indicating a moderate level of diversity.
The Shannon diversity index measures the diversity or evenness of a community by considering the relative abundance of different species. To calculate the Shannon diversity value for the given fish sample, we can use the formula:
H' = -Σ(\(\pi\) * log(\(\pi\)))
Where:
H' is the Shannon diversity value
\(\pi\) is the proportion of individuals belonging to the ith species
Using the data provided, we can calculate the Shannon diversity value as follows:
\(\pi\) for bluegill = 12/38
\(\pi\) for bass = 7/38
\(\pi\) for catfish = 6/38
\(\pi\) for carp = 13/38
Calculating the values and substituting them into the formula:
H' = -( (12/38 * log2(12/38)) + (7/38 * log2(7/38)) + (6/38 * log2(6/38)) + (13/38 * log2(13/38)) )
= 1.791
After performing the calculations, the Shannon diversity value for this sample of fish from the pond is approximately 1.791.
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The antlion is a ______
Explanation:
Antlion, (family Myrmeleontidae), any of a group of insects (order Neuroptera) that are named for the predatory nature of the larva, which trap ants and other small insects in pits dug into the ground. Antlions are found throughout the world, primarily in dry, sandy regions.
Explain why clumping hemoglobin proteins form long chains in sickle cell disease individuals
Answer:
Sickle hemoglobin exists as isolated units in the red cells when they have oxygen bound. When sickle hemoglobin releases oxygen in the peripheral tissues, however, the molecules tend to stick together and form long chains or polymers. These rigid polymers distort the cell and cause it to bend out of shape.
Explanation:
Select the correct location on the image.
The image shows towering clouds created by a low-pressure system. Which arrow correctly shows the primary air movement in this system?
Plssss help
Answer:
the answer is the up arrow
Explanation:
Answer:
out of the four arrows pointing at the cloud, the answer is the arrow pointing at the bottom of cloud
please help !! I don’t know the answer !!
Answer:
I am pretty sure it is A or D sorry if this does not help
_____ fermentation keeps __20_ going when oxygen is not present
Alcohol fermentation keeps going when oxygen is not present.
Alcoholic fermentation is a type of fermentation that occurs in the absence of oxygen, allowing cells to produce energy from glucose even when oxygen is not available. This process is used by some microorganisms, such as yeast, to convert sugars into alcohol and carbon dioxide.
During alcoholic fermentation, the first step is glycolysis, which breaks down glucose into two molecules of pyruvate, producing two ATP molecules. However, since oxygen is not present, pyruvate cannot enter the mitochondria for further processing through the Krebs cycle and electron transport chain.
Instead, the pyruvate is converted into ethanol and carbon dioxide through a series of enzymatic reactions.
The first step in alcoholic fermentation is the conversion of pyruvate into acetaldehyde, releasing a molecule of carbon dioxide in the process.
This reaction is catalyzed by the enzyme pyruvate decarboxylase. The acetaldehyde is then reduced to ethanol by the enzyme alcohol dehydrogenase, which uses NADH as a reducing agent.
This reaction regenerates NAD+ that is required for glycolysis to continue, allowing the cell to produce a small amount of ATP.
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Which best matches objects that a scientist would include on a list describing a forest ecosystem?
different types of animals and plants and the forest soil
O one animal species, the soil, water, and nutrients
o hall the living things in the forest ecosystem
O different types of soil, rocks, and water sources in the forest
Answer:
all the living things in the forest ecosystem
Describe the structure of large bones.
Answer:
Periosteum, Compact Bone, Spongy Bone, Bone Marrow, Articular Cartilage, Medullary Cavity
Explanation:
Periosteum: The outermost layer of a bone is called the periosteum. It is a tough, fibrous membrane that covers the bone's surface. The periosteum contains blood vessels, nerves, and connective tissue that nourish and support the bone.
Compact Bone: Beneath the periosteum lies a layer of compact bone, also known as cortical bone. Compact bone is dense and hard. It forms the main shaft or diaphysis of the long bone. Its structure consists of multiple layers of tightly packed mineralized matrix called lamellae, which contain collagen fibers. Compact bone provides strength and protection to the bone.
Spongy Bone: The interior of the bone, particularly at the ends and in the middle of long bones, contains spongy bone, also called cancellous or trabecular bone. Spongy bone has a porous, lattice-like structure composed of thin, branching bony plates called trabeculae. The spaces between the trabeculae are filled with bone marrow. Spongy bone helps reduce the weight of the bone while providing support and flexibility.
Bone Marrow: Within the spaces of spongy bone is bone marrow. There are two types of bone marrow: red marrow and yellow marrow. Red marrow is responsible for producing blood cells, including red blood cells, white blood cells, and platelets. Yellow marrow consists mainly of fat cells and serves as a storage site for adipose tissue.
Articular Cartilage: At the ends of long bones, where they articulate with other bones in joints, there is a layer of smooth, slippery cartilage called articular cartilage. It helps reduce friction and absorbs shock during movement, facilitating smooth joint motion.
Medullary Cavity: Within the diaphysis or shaft of the long bone, there is a hollow space called the medullary cavity. The medullary cavity contains bone marrow and serves as a storage site for yellow marrow.
A physical oceanographer studies all of the following except:
ete
of
Select one:
a. Currents
b. Waves
c. Tides
d. Fish
Check
Answer:
The answer is option d.
Fish
Hope this helps
Which scientific claim can be made? Songbird numbers will increase due to the effects of climate change increasing global temperatures. Songbirds appear more often in April and May. With an increase in construction, songbirds will move from one neighborhood to another. A study by Dr. Birde showed that the number of songbirds in her yard increased due to a modified food source.
The scientific claim that can be made is "A study by Dr. Birde showed that the number of songbirds in her yard increased due to a modified food source."
In Dr. Birde's study, it was observed that the presence of a modified food source resulted in an increase in the number of songbirds in her yard. This suggests a positive correlation between the availability of the modified food source and the population of songbirds. By analyzing the data collected in the study, Dr. Birde was able to draw a conclusion that the modified food source had a significant impact on attracting songbirds to her yard.
The modification of the food source may have included factors such as specific types of feed, feeding stations, or other environmental conditions that were favorable for songbird attraction. This finding indicates that altering the food source can potentially influence the presence and abundance of songbirds in a given area. Further research and replication of this study could provide valuable insights into songbird conservation and habitat management strategies.
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Conlan wants to answer the question, “Does phosphorus affect tomato fruit production?” Which statement represents a hypothesis that he can test to answer his question?
Provided question lacks the group of choices to answer the question, however, the correct group of choices are given below:
A)If phosphorus affects tomato fruit production, then tomatoes that are fertilized with phosphorus will have larger leaves.
B)If phosphorus affects tomato fruit production, then phosphorus should be added to the soil during planting.
C)If phosphorus affects tomato fruit production, then adding phosphorus to the soil will make tomatoes taste better.
D)If phosphorus affects tomato fruit production, then plants that are fertilized with phosphorus will produce more tomatoes.
Answer:
The correct answer would be option D) )If phosphorus affects tomato fruit production, then plants that are fertilized with phosphorus will produce more tomatoes.
Explanation:
To answer the question of Conlan, “Does phosphorus affect tomato fruit production?” he should form a hypothesis that can test the amount of the phosphorus and amount of the production of tomato fruits.
Productivity of fruits dealt with the producing tomato fruit instead of their taste, or size of leaves. So the statement D represent the hypothesis for his question due to the fact that phosphorus can increase the capacity of tomato fruit production.
Thus, the correct answer is option D.
4. Why were the humans able to develop agriculture at the beginning of the Holocene Period?
A. Woolly mammoth herds left behind large numbers of animal droppings for fertilizer.
B. The ice from the previous ice age melted and the climate was warmer.
C. Cave Men developed metal working technology that helped them make plows.
D. Holocene period men were able to select cold loving plants as crops.
The humans were able to develop agriculture at the beginning of the Holocene Period as the ice from the previous ice age melted and the climate was warmer. The correct option is B.
What is Holocene Period?The current geological epoch is the Holocene. It began approximately 11,650 years ago, following the Last Glacial Period, which ended with the Holocene glacial retreat.
The Quaternary period is comprised of the Holocene and the preceding Pleistocene.
Agriculture and civilization were made possible by the Holocene's unusually warm and stable climate.
Previously, year-to-year temperature and rainfall variations made agriculture too unreliable to support settled communities with large populations.
Thus, the correct option is B.
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Group the phrases according to the type of cell they describe.
Group 1 -Eukaryotic
Group 2 -Prokaryotic
Group 3 -Both
Options
1-Can have rigid cell walls
2-Genes float free cytoplasm
3-Contains organelles
4-Divides by mitosis
5-Genes enclosed in nucleus
6-Bacteria
Answer:
1. Both
2. Prokaryotic cells
3. Eukaryotic cells
4. Eukaryotic cells
5. Eukaryotic cells
6. Prokaryotic cells
Eukaryotic cells have true nuclei. Prokaryotic cells do not have a true nucleus.
What is eukaryotic cell?Eukaryotic cell have rigid cell walls. In prokaryotes the genes flow free in cytoplasm. Eukaryotes contain organelles. Prokaryotes do not contain organelles.
Eukaryotic cell divides by mitosis. In eukaryotic cell genes are enclosed in a nucleus. Bacteria are prokaryotic cell.
A prokaryotic cell is a straightforward, one-celled (unicellular) organism that is devoid of a nucleus or any other organelle that is membrane-bound. Soon, it will become clear that this is very different in eukaryotes. The nucleoid, a dark area in the center of the cell, is where prokaryotic DNA is located.
Therefore, Eukaryotic cells have true nuclei. Prokaryotic cells do not have a true nucleus.
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Which of the following organisms is a heterotroph? a) Rose b) Pine tree c) Deep-sea tubeworm d) None of these
Since the given organisms prepare their own food, hence none of the organisms mentioned are heterotrophs. Thus, the correct answer is Option D.
Heterotrophs are organisms that depend on other species (plants and animals) for food since they are unable to prepare it for themselves. They are dependent on these producers for food, either directly or indirectly.
The rose and the pine tree, two of the choices given, are both autotrophs since plants can make their own food through photosynthesis. On the other hand, the deep-sea tubeworm is a chemoautotroph, meaning it gets its energy from inorganic substances in its surroundings.
Therefore, the correct answer is Option D.
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identify a true statement about the pharmacological nature of mescaline.
The true statement about the pharmacological nature of mescaline is b. Tolerance develops more slowly to mescaline than to d-lysergic acid diethylamide (LSD).
Mescaline, a psychedelic substance, can result in hallucinations and altered states of consciousness. This is due to the significant changes in sensory and perceptual experiences that it causes. According to research, mescaline tolerance grows more gradually than LSD tolerance. This implies that over time, recurrent mescaline usage may necessitate higher dosages of the drug to maintain the same benefits.
Alcoholism, depression, and other mental illnesses are all treated with it. Some hallucinogens are obtained from plants or mushrooms, while others are artificial, or man-made. Previously, individuals utilised hallucinogenic substances for therapeutic or religious purposes, but more recently, they are used for social and recreational activities.
Complete Question:
Identify a true statement about the pharmacological nature of mescaline.
a. It is the treatment of posttraumatic stress disorder
b. Tolerance develops more slowly to mescaline than to d-lysergic acid diethylamide (LSD).
c. It produces alterations of visual, auditory, and tactile perception.
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what conclusion can you draw abou the inheritance of these traits based on the f1 results
Based on the F1 results, we can conclude that both traits are inherited independently, meaning that the occurrence of one trait does not affect the occurrence of the other.
We can also conclude that the alleles of both traits show a 1:1 ratio in the F1 generation. This means that there is a 50% chance that the offspring will receive either allele from the parent plants.
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An organ that develops a secretion is a/an __________.
A. estrogen B. hormone C. gland D. progesterone
2. A _____________ is the chemical secretion of endocrine glands.
A. blood B. gland C. hormone D. vessel
3. The following are the functions of the endocrine system EXCEPT for ________.
A. mental development C. sexual development
B. physical development D. spiritual development
4. The gland that controls all the endocrine glands is the _________.
A. hypothalamus B. pituitary gland C. thalamus D. thyroid gland
5. The major glands in the body are the following EXCEPT for ______.
A. pituitary B. parathyroid C. thyroid D. thalamus
6. Which gland regulates the body metabolism?
A. pituitary B. parathyroid C. thyroid D. thalamus
Which of the following controls maturation and the male characteristics?
A. adrenal B. ovaries C. pancreas D. testes
8. The ________influence female traits and support reproductive function.
A. adrenal B. ovaries C. pancreas D. testes
9. This plays an important role in both male and female reproductive systems.
A. estrogen B. hormone C. gland D. progesterone
10. Hormones can cause a series of chemical reactions within __________.
A. cell B. glands C. tissues D. target organs
11. Which of the following is known as the emergency hormone?
A. adrenalin B. androgen C. estrogen D. progesterone
12. It regulates the blood sugar level.
A. pancreas B. parathyroid C. pituitary D. thyroid
13. Which part enables the body to produce certain antibodies?
A. pituitary B. parathyroid C. thyroid D. thymus
14. Which of the following glands controls the calcium levels in the body and
normalizes bone growth?
A. pituitary B. parathyroid C. thyroid D. thymus
15. This contains a group of glands that releases hormones in the body.
A. Circulatory System C. Nervous System
B. Endocrine System D. Respiratory System
Answer: 1. C. 2. C. 3.D 4.B 5.D 6.C 7.D 8.B 9.B 10.D 11.A 12.A 13.D 14.B 15.B
Explanation:
The correct blanks are as follows:
1. Glands
2. Hormones
3. Spiritual development
4. Pituitary gland
5. Thalamus
6. Thyroid
7. Testes
8. Ovaries
9. Hormone
10. Target organs
11. Adrenalin
12. Pancreas
13. Thymus
14. Thymus
15. Endocrine System
What is an endocrine gland?
An organ that makes hormones that are released directly into the blood and travel to tissues and organs all over the body.
Endocrine glands help control many body functions, including growth and development, metabolism, and fertility.
Thus, the correct blank is as follows.
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choose all the answers that apply
without decomposers:
chemical nutrients would not return to the soil
dead organisms would pile up
the sun's energy could not be used
matter would not be recycled
an ecosystem is unbalanced
MULTIPLE CHOICE!!
Here are the correct answers from Odyessyware! I got 100% correct, so the answers are: dead organisms would pile up, and matter would not be recycled.
Answer:
chemical nutrients would not return to the soil
dead organisms would pile up
matter would not be recycled
an ecosystem is unbalanced
Explanation:
Which feature of the model represents stored chemical energy
The sugar molecule represents the most stored chemical energy. Thus, the correct answer is Option B.
Sugar (glucose, C₆H₁₂O₆) is a biological molecule that stores energy in its chemical bonds, not the molecule that initiates the process of cellular respiration During cellular respiration, glucose is broken down in a series of chemical reactions to produce energy ATP (adenosine triphosphate).
The process of cellular respiration begins at the stage of glycolysis, where glucose in the cytoplasm of the cell is partially broken down into pyruvate molecules and then the pyruvate molecules are broken down again in the presence or production of oxygen (aerobic respiration). fermentation in the absence of oxygen depending on whether oxygen is present
During aerobic respiration, pyruvate enters mitochondria and undergoes the citric acid cycle (also known as the Krebs cycle) and the electron transport chain These processes release energy from glucose chemical bonds to produce more ATP.
Therefore, sugar (glucose), although it stores energy in its chemical bonds, is not the molecule that initiates cellular respiration. Instead, during cell respiration, glucose is broken down and energy is released in the form of ATP.
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Complete question:
Which feature of the model represents the most stored chemical energy?
A. The oxygen gas molecule
B. The sugar molecule
C. The water molecule
D. The carbon dioxide molecule
"Please indicate whether each phrase or acid is describing a saturated fatty acid, monounsaturated fatty acid, or polyunsaturated fatty acid. (12 points) Your answers must be spelled correctly for full credit.
Each carbon is bonded with two atoms of hydrogen
Oleic acid
Double bond occurs at only one point in the carbon chain
Alpha-Linolenic acid
Contains more than one double bond
Stearic acid"
Each carbon is bonded with two atoms of hydrogen: Saturated fatty acid.
- Oleic acid: Monounsaturated fatty acid.
- Double bond occurs at only one point in the carbon chain: Monounsaturated fatty acid.
- Alpha-Linolenic acid: Polyunsaturated fatty acid.
- Contains more than one double bond: Polyunsaturated fatty acid.
- Stearic acid: Saturated fatty acid.
Saturated fatty acids have each carbon atom in the carbon chain bonded to two hydrogen atoms, meaning there are no double bonds present. Therefore, the phrase "Each carbon is bonded with two atoms of hydrogen" describes a saturated fatty acid.
Oleic acid is a monounsaturated fatty acid because it has one double bond occurring at a specific point in the carbon chain. The phrase "Double bond occurs at only one point in the carbon chain" accurately describes oleic acid.
Alpha-Linolenic acid is a polyunsaturated fatty acid because it contains more than one double bond. The phrase "Contains more than one double bond" correctly describes alpha-Linolenic acid.
Stearic acid, on the other hand, is a saturated fatty acid because it lacks any double bonds. Therefore, the phrase "Contains more than one double bond" does not apply to stearic acid.
By identifying the presence or absence of double bonds, we can categorize the given fatty acids as saturated, monounsaturated, or polyunsaturated.
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What amount of force needs to be applied to a 20 kg bowling ball to give it an acceleration of 5 m/s2? Bubble your answer on your bubble sheet.
Answer: 100N
Explanation: Given parameters:
Mass of the bowling ball = 20kg
Acceleration = 5m/s²
Unknown:
Amount of force applied = ?
Solution:
To solve this problem, we apply newton's second law of motion.
Force = mass x acceleration
Now insert the parameters and solve;
Force = 20 x 5 = 100N
2
What is a hypothesis?
OA.
a statement that summarizes the results of an experiment
OB.
a question that cannot be scientifically tested
OC.
a description of the steps involved in an experiment
OD
a statement that can be tested through a scientific investigation
Reset
Submit
Answer:
D
Explanation:
a statement that can be tested through a scientific investigation
Answer:
D
Explanation:
the answer is d because a hypothesis is an assumption that will be tested in the expirement.
Do neurons belong to the central nervous system or the peripheral nervous system
Answer:
the nervous system