The protease papain, which can be isolated from papaya fruit, is a monomer with a single active site per protein molecule. The activity of purified papain was measured at various substrate concentrations and plotted on the Lineweaver burk plot below. Considering that the enzyme concentration is 1.2 x 10-7 M, answer the following questions 35 30 25 20 15 10 10 5 0 5 2025, 30 mM) Substratel A) Calculate Km for this substrate B) Calculate Vmax C) Calculate Keat D) Based on the Kea/ Km is this a good substrate for the enzyme E) What fraction of the total enzyme has substrate bound when the substrate concentration 2.5 x 104 M?

Answers

Answer 1

Km for this substrate Is 2 mM. Vmax of the substance is 14.9995 mM/s. The value of Kea for this enzyme is approximately 1.249 x 10^6 M^-1*s^-1. For this enzyme kea to km is  very high ratio, indicating that the enzyme is a good substrate for the enzyme.

To calculate Km from a Lineweaver-Burk plot, you need to find the substrate concentration at which the reciprocal of the substrate concentration (1/[S]) is equal to the reciprocal of the initial velocity (1/V0). This point is called the point of inflection on the Lineweaver-Burk plot. In this case, the point of inflection is located at a substrate concentration of approximately 20 mM. At this point, the reciprocal of the substrate concentration is 1/[S] = 1/20 mM = 0.05, and the reciprocal of the initial velocity is 1/V0 = 0.1. To calculate Km, you can use the equation: Km = (1/V0) / (1/[S]) Plugging in the values, you get: Km = (1/V0) / (1/[S]) = 0.1 / 0.05 = 2 mM The value of Km for this substrate is 2 mM. To calculate Vmax, you can use the equation: Vmax = V0 / (1 + Km/[S]). Where Vmax is the maximum initial velocity, V0 is the initial velocity, Km is the Michaelis constant, and [S] is the substrate concentration. Aa substrate concentration of 2.5 x 10^4 mM, the initial velocity is approximately 15 mM/s. Plugging in the values, you get: Vmax = 15 mM/s / (1 + 2 mM / 2.5 x 10^4 mM) = 15 mM/s / (1 + 8 x 10^-4) = 15 mM/s / 1.00008 = 14.9995 mM/s The value of Vmax for this enzyme is approximately 14.9995 mM/s. To calculate Kea, you can use the equation: Kea = Vmax / [E] Where Kea is the catalytic constant, Vmax is the maximum initial velocity, and [E] is the enzyme concentration. Plugging in the values, you get: Kea = 14.9995 mM/s / (1.2 x 10^-7 M) = 1.249 x 10^6 M^-1*s^-1 The value of Kea for this enzyme is approximately 1.249 x 10^6 M^-1*s^-1. The ratio of Kea to Km is a measure of the efficiency of the enzyme-substrate complex. A high ratio indicates that the enzyme is a good substrate for the enzyme, while a low ratio indicates that the enzyme is a poor substrate for the enzyme. In this case, the ratio of Kea to Km is approximately 1.249 x 10^6 M^-1s^-1 / 2 mM = 6.245 x 10^4 M^-1s^-1. This is a very high ratio, indicating that the enzyme is a good substrate for the enzyme.

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Related Questions

How many moles of MgS are in 1.00g MgS?

Answers

Answer:

24.31 g/mol.

Explanation:

moles =mass/molar mass

n=w/m

can u pls help me i need the answer ASAP

can u pls help me i need the answer ASAP

Answers


The answer is c ( to disturbance heat

What is the mass in grams of 0.6 mol C4,H10?

Answers

Please check the attached picture
What is the mass in grams of 0.6 mol C4,H10?

The mass of the 0.6 mol of the butane C₄H₁₀ is equal to 34.8 g.

What is a mole?

A mole can be described as a standard unit that can be used to calculate a given count of particles. The particles can be usually chemically identical entities, individually distinct.

A mole can be used to measure a huge number of quantities of atoms, molecules, or other particular particles. The number of chemical substances can be explained as a measure of elementary entities.

The number of particles present in one mole is 6.023 × 10 ²³ which is Avogadro’s number.

Given, the number of moles of butane = 0.6 moles

The mass of one mole of butane = 58 g

Then, the mass of 0.6 moles of butane = 0.6 × 58

The mass of 0.6 moles of butane = 34.8 g

Therefore,  34.8 grams is the mass of 0.6 moles of butane.

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What is the molar concentration (molarity, M) of a 450 mL solution that contains 2.00 mol of FeCl₂?​

Answers

Answer:

3.50 Mol/L or M

Explanation:

450 mL / 1000 = 0.45 L

1 mole ------- 126.751 g

? mole ------- 200 g

moles = 200 * 1 / 126.751

moles = 200 / 126.751

= 1.57789 moles FeCl₂

M = n / V

M = 1.57789 / 0.45

= 3.50 Mol/L or M

What is the net ionic equation for the reaction shown below?

What is the net ionic equation for the reaction shown below?

Answers

A net ionic equation includes the ions that are involved in the formation of the solid.

In this case, the solid is silver chloride (AgCl). The ions that are involved are Ag+ and Cl-, then, the net ionic equation is:

\(Ag_{(aq)}^++Cl_{(aq)}^-\rightarrow AgCl_{(s)}\)

It means that the correct answer is the last choice.

What is a hot spot? Must be in your own words ( please hurry)

Answers

Answer:

It depends. what hot spot are you looking for?

Answer:

A hot spot is an area on Earth over a mantle plume or an area under the rocky outer layer of Earth, called the crust, where magma is hotter than surrounding magma. The magma plume causes melting and thinning of the rocky crust and widespread volcanic activity.

Explanation:

Hope this is what you mean be hot spot!

I hope this helps you!  

Write a net ionic equation for the reaction that occurs when aqueous solutions of hydrochloric acid and sodium hydroxide are combined.

Write a net ionic equation for the reaction that occurs when aqueous solutions of hydrochloric acid and

Answers

HCl +NaOH = NaCl + H20.

What is the SI unit that refers to the number of particles contained in a substance

Answers

The SI unit that refers to the number of particles contained in a substance is the mole (mol).

multiplying by which conversion factor would allow you to convert 35 liters to milliliters​

Answers

Multiplying by 1000 will allow you to convert 35 liters to milliliters

Conversion scale

1 Liters = 1000 milliliters

Thus, multiply the value in liter by 1000 will convert it to milliters

With the above scale, we can convert 35 liters to milliliters. Details below:

How to convert 35 liters to milliliters

We can convert 35 liters to milliliters as illustrated below:

1 Liters = 1000 milliliters

Therefore,

35 liters = (35 liters × 1000 milliliters) / 1 liters

35 liters = 35000 milliliters

Thus, 35 liters is equivalent to 35000 milliliters

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Convert 100.6 Kelvin to degrees C.

°C = K - 273
[?] °C ​

Answers

Answer:

  -172.6 °C

Explanation:

You want to know the Celsius equivalent of the temperature 100.6 K.

Conversion

The relation is ...

  C = K - 273.15

  C = 100.6 -273.15 = -172.55

The temperature is -172.55 °C, about -172.6 °C.

__

Additional comment

We have rounded to tenths, because that is precision of the temperature given. If you use 273 as the conversion constant, you will get -172.4.

what is 1.01 km to mm in scientific notation​

Answers

Answer:

= 3.456 × 1011

Explanation:

Select all the correct answers.
Which statements about energy are true?
Energy is found only in moving objects.
Energy is never created.
Energy can move matter.
Energy is the same as force.
Energy is never destroyed.
Submit

Answers

Answer:

Energy is found only in moving objects, Energy can move matter, Energy is never destroyed

Energy is neither created nor destroyed. Therefore, option (B), (E) are correct.

What is the law of conservation of energy?

According to the law of conservation of energy, the energy can only be changed from one to another but can not be created or destroyed. The energy present in nature in several forms such as nuclear energy, heat, electricity, chemical energy, and so on.

The total energy of an isolated system remains constant when all forms of energy are considered. This law of energy is applicable to all kinds of energy.

In an isolated system, the loss of energy in one area, there will be a gain of energy in another section. There is no known case where this law is violated.

In mechanics, the fundamental quantities which are conserved are energy, momentum, and angular momentum. A small amount of energy is lost and energy transformation takes place although energy does not actually disappear, it converts into other unusable forms.

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HELP PLEASE I GIVE BRAINLIEST

HELP PLEASE I GIVE BRAINLIEST

Answers

Note that the option that the BEST claim based on the given evidence above is: " "Turbo-Grow plant food is the best fertilizer for making plants grow tall." This is because from the evidence, Turbo-Grow made plants grow the tallest to the height of 40cm

What is claim and evidence and how are they related to experiments?

A claim is a statement or conclusion that is put forth as true, while evidence is the supporting data, observations, or facts that back up that claim.

In experiments or research, the claim is often the hypothesis, which is tested through data collection and analysis. The evidence gathered through the experiment or research is then used to support or refute the initial claim or hypothesis.

Strong evidence can lend credibility to a claim, while weak or conflicting evidence can undermine it. It is important for claims to be supported by strong, relevant evidence in order to draw accurate conclusions from the research or experiment.

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Solar and wind energy are both intermittent resources that cannot be relied upon for a constant stream of energy production. Explain why developing better ways to store energy is an important part of making these energy sources more practical to use.

Answers

By removing the need to build additional transmission lines and equipment, energy storage may reduce costs for utilities and their customers.

By removing the need to build additional transmission lines and equipment, energy storage may reduce costs for utilities and their customers. Energy storage's inherent ability to offer backup power in the event of grid failure is a feature that both residential consumers and commercial owners find highly desirable.

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Write an equation that shows the formation of the bromide ion from a neutral bromine atom.

Answers

The equation that shows the formation of the bromide ion from a neutral bromine atom is as follows: Br + e- → Br-

What is an ion?

Ion is the atom or group of atoms bearing an electrical charge, such as the sodium and chlorine atoms in a salt solution.

An atom becomes an ion when it loses or gains electrons.

According to this question, bromine atom is neutrally charged but quicky becomes charged into an ion when it gains one electron. The equation is represented as follows:

Br + e- → Br-

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5500 J of heat were added to three moles of a monoatomic gas. During this process the temperature decreased from 540 K to 350 K. What is the work performed by the gas during this process

Answers

Answer:

The work performed ≅ 1.26 × 10 ⁴ J

Explanation:

The internal energy change of a monoatomic ideal gas is represented by the equation:

ΔU = n*Cv*ΔT

where;

the heat capacity Cv for an ideal monoatomic gas is (3/2) R

Then;

ΔU = n × (3/2) R × ΔT

ΔU = 3 × (3/2) × 8.314 ×( 540-350)

ΔU = 7108.47 J

Using the 1st law of thermodynamics;

The work performed = Heat added + the internal energy change

The work performed = 5500 J + 7108.47 J

The work performed = 12608.47

The work performed ≅ 1.26 × 10 ⁴ J

What is the volume of nitrogen dioxide (in L) is produced from the complete reaction of 16.87g of lead (II) nitrate at STP?

Answers

Given the reaction N2 + 3H2 -->2NH3, what volume of hydrogen is necessary to react with five liters of nitrogen to produce ammonia, assuming constant temperature and pressure? How would you calculate the mass in grams of hydrogen chloride produced when 4.9 L of molecular hydrogen at STP reacts with an excess of chlorine gas.?

What ideas do you have about why Christchurch’s air temperature is cooler during el niño years?

Answers

One possible reason for cooler air temperatures in Christchurch during El Niño years is the shift in atmospheric circulation patterns.

During El Niño years, Christchurch may experience cooler air temperatures due to several factors associated with the El Niño phenomenon.

El Niño is characterized by the abnormal warming of the surface waters in the eastern tropical Pacific Ocean, which has global climatic implications. While El Niño is primarily associated with changes in oceanic conditions, its effects can extend to atmospheric patterns, leading to altered weather patterns and temperature variations.

One possible reason for cooler air temperatures in Christchurch during El Niño years is the shift in atmospheric circulation patterns. El Niño can disrupt the normal global atmospheric circulation, resulting in changes in the positioning and intensity of weather systems.

This can lead to the advection of cooler air masses from the south or southeast towards Christchurch, resulting in cooler temperatures.

Another factor is the influence of El Niño on regional rainfall patterns. El Niño often leads to drier conditions in the South Island of New Zealand, including Christchurch.

Reduced cloud cover and less moisture in the air can contribute to cooler temperatures as there is less insulation from the sun's radiation and less evaporative cooling. Additionally, the absence of significant rainfall can result in less moisture in the soil, leading to cooler conditions as less energy is used for evaporation.

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Given the reaction at equilibrium:
2NO2(g) → N204(g) Heat of
reaction is -55.3 kJ) What type of
reaction is this?
O Endothermic
O Exothermic

Answers

When the equilibrium constant is higher than one, it indicates that the reaction prefers to produce products, whereas if the equilibrium constant is less than one, it indicates that the reaction prefers to produce reactants. If the equilibrium constant is equal to one, the reaction proceeds in both directions equally.

In a chemical reaction, exothermic reactions are defined as reactions that release heat into their environment. It implies that heat is given off when reactants are converted to products. At equilibrium, an exothermic reaction continues to be exothermic, meaning that heat is given off even after the reaction reaches a state of equilibrium.There are two types of reactions: exothermic and endothermic.

A reaction is classified as exothermic if it releases heat, and endothermic if it absorbs heat. The direction of the reaction is determined by whether it is exothermic or endothermic. At equilibrium, the reaction is no longer moving forwards or backwards. It's also worth noting that reactions can be exothermic in one direction and endothermic in the other.

The equilibrium constant (K) is defined as the ratio of the concentration of products to the concentration of reactants in the chemical reaction equation. It is used to express how much of the products is generated by the reaction in comparison to the reactants.  the equilibrium constant aids in the identification of the direction in which the reaction will proceed at equilibrium.

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[Co(H2O)6]2+ what is the name

Answers

try hexaaquacobalt(II) cation

A sample containing 16.93 metal pellets is poured into a graduated cylinder containing 11.2 of water, causing the water level in the to 19.7 Calculate the density of the metal.

Blank = g/ml

Answers

Answer:

The answer is 1.99 g/mL

Explanation:

The density of a substance can be found by using the formula

\(density = \frac{mass}{volume} \\\)

From the question

mass = 16.93 g

volume = final volume of water - initial volume of water

volume = 19.7 - 11.2 = 8.5 mL

We have

\(density = \frac{16.93}{8.5} \\ = 1.9917647058...\)

We have the final answer as

1.99 g/mL

Hope this helps you

Why are the united nation members upset with Wakanda?

Answers

The "Wakanda speech," six weeks have passed, and the world is still in shock.

Thus, Global leaders and analysts were taken aback by King T'Challa's declaration at the United Nations General Assembly that the Kingdom of Wakanda is not a developing country of textiles, farms, and shepherds with a GDP per person of roughly $760 but rather a technological superpower and Wakanda speech.

The country's widespread employment of cutting-edge magnetic levitation trains, flying machines, opaque holograms, and spinal cord-healing beads has led to the coining of the phrase "uber-developed" nation.

The most watched video ever is currently "Welcome to the Future," an introduction video created by Wakanda's recently established Ministry of Foreign Affairs.

Thus, The "Wakanda speech," six weeks have passed, and the world is still in shock.

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Which of these shows the correct hierarchical sequence?
organs cells ▸ tissues ► organ systems
cells ► tissues ► organs organ systems
organ systems tissues › cells organs
tissues cells organs ► organ systems

Answers

Answer:

cells -> tissues -> organs -> organ system

Answer:

Cells > tissues > Organs > Organs Systems

Explanation:

tissues are made of a group of cells

a specific organ is made up of a geoup of specific type of tissues

and a group of organs make up the organs system

1. What is the concentration of NaCl (salt) when 4.9 moles are dissolved in 7 liters
of water?

Answers

The concentration of NaCl (salt) when 4.9 moles are dissolved in 7 liters of water is 34.3M

Molarity is a unit of concentration measuring the number of moles of a solute per litre of solution.

Chemists primarily need the concentration of solutions to be expressed in a way that accounts for the number of particles that react according to a particular chemical equation. Since percentage measurements are based on either mass or volume, they are generally not useful for chemical reactions. A concentration unit based on moles is preferable.

Given,

Moles of NaCl = 4.9 moles

Volume = 7 L

Concentration = moles × volume (L)

= 34.3 M

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The aromatic region (7-8 ppm) of the proton NMR spectrum of compounds with para disubstituted benzene rings such as phenacetin is often referred to as an AB pattern. This pattern has two doublet signals coupled to each other. Explain the origin of this AB pattern.

Answers

The aromatic ring in phenacetin exhibits an AB pattern because it has two mutually coupled nuclei that are unrelated to other protons or whose shift differences is equal to the coupling constant.

Why are protons present on deshielded benzene?

Deshielding occurs for the ring protons of benzene due to their larger chemical shift (7.3 ppm vs. 5.6 ppm for the vinylic protons in cyclohexene) and the fact that the magnetic field induced outside of the ring is the same orientation as that of the external field.

What frequency band does benzene proton NMR display?

Protons are present in benzene at levels of 6–8 ppm.This value is larger than that of the protons in those other hydrocarbon like alkanes & alkenes because of the aromatic ring's deshielding effect.

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4. The volume of a liquid sample is measured as 15.43 L. We need to know the volume in
mL.
3.
b.
What conversion factor would be used in the calculation?
Calculate the volume in mL.

Answers

Conversion factor used to convert volume is 1L = 1000 mL.

15.43L = 15430mL

The same attribute is expressed using a unit conversion but in a different unit of measurement. For instance, time can be expressed in minutes rather than hours, and distance can be expressed in kilometers, feet, or any other measurement unit instead of miles. Measurements are frequently offered in one set of units, like feet, but are required in another set, like chains. A conversion factor is a mathematical equation that facilitates an equal exchange of feet for chains.

A conversion factor is a number that is used to multiply or divide one set of units into another. If a conversion is required, it must be done using the correct conversion factor to get an identical value.

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Homeostasis in a constantly changing environment is a process known as

Answers

The Excretory system

What happens to metals when they form ionic bonds?

Answers

Answer:

The metal atoms become positive ions and the non-metal atoms become negative ions. There is a strong electrostatic force of attraction between these oppositely charged ions – this is called an ionic bond.

Explanation:

A Student records the amount of sunlight that a plant receives each day for one week
Which ope of energy transfer is also being recorded?

convection
Radiation
conduction
rotation

Answers

The mode of light passing from sun to earth is an example of radiation. The student thus recording the energy transfer mode is radiation and option b is correct.

What is radiation?

There are three modes of energy transfer namely, conduction, convection and radiation.  Conduction is the process of energy transfer takes place in solids where, energy is transferred through a chain of closely packed molecules.

Convection is the energy transfer mode in liquids where, the energetic molecules spreads the energy up to the surface by moving above and thus transferring the energy in all directions.

Radiation is the energy transfer mode takes place in gases. The light passing through the air is an example of radiation. All types of energy transfer in space and in the air are radiations.

Therefore, the recorded energy transfer mode is radiation and option B is correct.

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Which equation shows an increase in entropy?
Hint: Look at the states of matter, g s l, of the chemicals in each equation. A C2H4(g) + H2(g) + C2H6(g) в Caco3(9) + Cao(s) - CO2(g) c Fe(s) + S (s) -+ FeS (s)

Which equation shows an increase in entropy?Hint: Look at the states of matter, g s l, of the chemicals

Answers

The equation C2H4(g) + H2(g) + C2H6(g) → Caco3(s) + Cao(s) + CO2(g) shows an increase in entropy due to the formation of a gas as a product. Option A

In this equation, the reactants on the left-hand side consist of gases (C2H4 and H2), while the products on the right-hand side include a solid (Caco3) and a gas (CO2).

When a reaction involves a change from gaseous to solid or liquid states, there is typically a decrease in entropy because the particles become more ordered and constrained in the solid or liquid phase.

Conversely, when a reaction involves the formation of gases, there is generally an increase in entropy because gases have higher degrees of molecular motion and greater freedom of movement compared to solids or liquids.

In the given equation, the reactants include three gaseous compounds (C2H4, H2, and C2H6), and one of the products is a gas (CO2). Therefore, the overall entropy of the system increases during this reaction.

The equation Fe(s) + S(s) → FeS(s) does not show an increase in entropy. Both the reactants (Fe and S) and the product (FeS) are solids. Since solids have lower entropy compared to gases or liquids, the entropy of the system does not increase in this reaction. Option A

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