When dna fragments are separated using gel electrophoresis and transferred to a blot it is called a:____.

Answers

Answer 1

When DNA fragments are separated using gel electrophoresis and transferred to a blot it is called a southern blot.

In the field of molecular biology, we can define southern blot as a method through which a particular DNA fragment is detected from amongst a DNA sample.

The method of southern blotting has important uses such as the detection of diseases, for finding out a DNA segment in a DNA library.

In the method of southern blotting, the DNA sample is first cut by using restriction enzymes. Then the sample is run by the method of gel electrophoresis due to which the smaller segments move at a faster speed than a larger one along a matrix. The DNA segments are then transferred to a blot. The DNA is marked by a tag, referred to as a probe, using radioactive, fluorescent or any other technique. This tag will allow the detecting of our desired DNA segment when a complementary segment is run referred.

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Related Questions

during the replication process how is the supercoiling DNA corrected?

Answers

The level of DNA supercoiling can be altered either by breaking-rejoining reactions that change the DNA linking number or by environmental changes that alter the helical pitch of DNA.

Urgent!!!! Worth 20 points!! Please help ASAP! It’s timed!! Only serious answers bad answers will be reported

Urgent!!!! Worth 20 points!! Please help ASAP! Its timed!! Only serious answers bad answers will be reported

Answers

It's a and d.
If treated with an acid they die.
And change in shape is Denaturing, because it means the enzym is being destroyed

State one example of the "Tragedy of the Commons”

Answers

Answer:

The tragedy of the commons is often brought up when people discuss environmental issues. For example, in fishing, if fishing provides an income, then each fisher would have his or her own best interest in mind and try to catch as many fish as possible even if all the other fishers are doing the same thing.

Explanation:

structure of leaf is complementary to its function explain

Answers

The leaf has a thin lamina, mid rib and network of veins. The large surface area of the lamina helps in the absorption of light and its thin nature minimizes the diffusion process for gaseous exchange.Hope this will help

Answer:

All leaves have the same basic structure - a midrib, an edge, veins and a petiole. The main function of a leaf is to carry out photosynthesis, which provides the plant with the food it needs to survive. Plants provide food for all life on the planet.

Explanation:

A patient presents with memory loss and partial paralysis. There is a syndrome with these symptoms. Metabolically speaking, what is the cause of this? The malfunctioning enzyme has lost affinity for a cofactor. The malfunctioning enzyme has lost affinity for its substrate. The malfunctioning enzyme is absent from the patient. The malfunctioning enzyme has gained affinity for its substrate

Answers

The cause of memory loss and partial paralysis is likely due to a syndrome. Metabolically speaking, the cause is likely that the malfunctioning enzyme has lost affinity for a cofactor. The correct answer is option a.

This can result in a decrease in the enzyme's activity, which can have detrimental effects on various metabolic pathways.

The other options presented in the question are not likely to be the cause of the syndrome.

If the enzyme has lost affinity for its substrate, it may have a decreased ability to convert the substrate to the product. However, this would not necessarily lead to the given symptoms.

If the enzyme is absent from the patient, it would result in a complete loss of activity for the enzyme, which may have more severe effects than the given symptoms. If the enzyme has gained affinity for its substrate, it may result in an increase in activity.

However, this is unlikely to be the cause of the given symptoms.

The correct answer is option a.

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Complete question

A patient presents with memory loss and partial paralysis. There is a syndrome with these symptoms. Metabolically speaking, what is the cause of this?

a. The malfunctioning enzyme has lost affinity for a cofactor.

b. The malfunctioning enzyme has lost affinity for its substrate.

c. The malfunctioning enzyme is absent from the patient.

d. The malfunctioning enzyme has gained affinity for its substrate

HURRY PLEASE I RLLY NEED THIS FOR THE MOST IMPORTANT TEST IM TAKING THIS YEAR. The diagram shows a toy car on a number line.

A line with arrowheads at both ends has ticks marks every 2 units. The tick marks for 0, 6, and 10 are labeled. A car sits above the tick mark for 2 with a dashed red line leading to the tick mark.
What is the position of the car?

–4
1
2
4

HURRY PLEASE I RLLY NEED THIS FOR THE MOST IMPORTANT TEST IM TAKING THIS YEAR. The diagram shows a toy

Answers

Answer:

The position of the car is +2 or simply 2

Explanation:

The position of an object is usually given from a reference position.

A number line is used to specify the position of the car in the given question.

A number line has numbers arranged on a straight line with positive and negative numbers found on both sides of zero serving at the center of the number line. Positive numbers are found to the right of zero on the number line while negative numbers are found to the left of zero onnthe number line.

In the given question, the number line has ticks marks every 2 units.

The car sits above the tick mark for 2 with a dashed red line leading to the tick mark.

Since the car is found to the right of zero on the number and sits above the tick mark for 2, the position of the car is +2 or simply 2


what happens in the NEXT stage of mitosis

Answers

Answer:

Cytokinesis

Explanation:

Cytokinesis happens after mitosis. It is the final division to create two new cells. In this phase, the cytoplasm divides and two complete daughter cells are created. These two new cells have genetic information that is identical to the parent cell from which they originated.

Answer:

Cytokinesis as the Next Step

Cytokinesis happens after mitosis. It is the final division to create two new cells. In this phase, the cytoplasm divides and two complete daughter cells are created. These two new cells have genetic information that is identical to the parent cell from which they originated.

Vhy did the color of the peppered moth change?
A.The moths' life cycle changed,
B.The environment changed
C.The amount of variation in the moth population changed
D.The number of individual moths in the population changed,

Vhy did the color of the peppered moth change?A.The moths' life cycle changed, B.The environment changedC.The

Answers

Answer:

the environment changed

Explanation:

The evolution of the peppered moth is an evolutionary instance of directional colour change in the moth population as a consequence of air pollution during the Industrial Revolution. ... Later, when pollution was reduced, the light-coloured form again predominated.

Answer:

B.The environment changed

Explanation:

Which of the following statements about water are true?

Select all correct answers.

A. The oxygen in water carries a potential negative charge.

B. Water is electrically neutral overall.

C. Water is made up of two atoms.

D. Water is not polar.

E. Water has unevenly distributed electric charges.

Answers

D. Water is not polar

Difference between eukaryotic cells and prokaryotic cells.​

Answers

I hope this helps you

........

Difference between eukaryotic cells and prokaryotic cells.

Answer:

eukaryotic cells are those which have a membrane-bound nucleus that contains genetic material, as well as organelles that are also membrane-bound. Whereas, prokaryotes are cells that don’t have a nucleus or membrane-encased organelles.

Explanation:

hope it will help you !

what is the resulting amino acid change at position 557 of the taster allele?

Answers

The resulting amino acid change at position 557 of the taster allele is PAV. The AVV allele codes for the valine amino acid at position 262 and the alanine amino acid at positions 49 and 296.TAS2R38 AVI/AVI individuals are PTC non-tasters, PAV/PAV individuals are PTC super-tasters, and AVI/PAV individuals are PTC medium tasters.

The resulting amino acid change at position 557 of the taster allele is PAV (Proline-Alanine-Valine). Taster alleles control the sensitivity to the bitter taste of the compound phenylthiocarbamide (PTC) and other related substances such as 6-n-propylthiouracil (PROP).The difference in perception of these compounds is due to variations in the genes that encode taste receptors.

TAS2R38 gene is the principal gene that encodes PTC taster status. TAS2R38 gene has three common variants: AVI, PAV, and AVV. A change in the amino acid sequence of the protein that is encoded by the TAS2R38 gene is responsible for the variation in the receptor’s function.The AVI allele codes for the proline, alanine, and valine amino acids at positions 49, 262, and 296, respectively. The PAV allele codes for the amino acids alanine, valine, and isoleucine at these positions.

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the type of selection that favors one homozygous type and is least favorable for the other homozygous type is question 9select one: a. stabilizing selection b. directional selection c. disruptive selection clear my choice

Answers

The type of selection that favors one homozygous type and is least favorable for the other homozygous type is C) disruptive selection

Disruptive selection occurs when extreme phenotypes have higher fitness than individuals with intermediate phenotypes. This can lead to the splitting of a population into two distinct groups, each with a different homozygous genotype.

In disruptive selection, individuals with one homozygous genotype are favored in one extreme of the phenotypic range, while individuals with the other homozygous genotype are favored in the opposite extreme.

This results in a decrease in the frequency of individuals with the intermediate homozygous genotype.

For example, imagine a population of butterflies where the color of their wings determines their survival and reproductive success.

If the environment contains two distinct habitats, one with light-colored flowers and the other with dark-colored flowers, butterflies with light-colored wings and butterflies with dark-colored wings will have higher fitness than butterflies with intermediate wing colors.

This would lead to disruptive selection, favoring the two extreme homozygous genotypes for wing color.

Stabilizing selection (option a) and directional selection (option b) both favor individuals with intermediate phenotypes, rather than extreme homozygous types.

Thus, the type of selection that favors one homozygous type and is least favorable for the other homozygous type is C) disruptive selection.

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true or false mutations sometimes result in a non -adaptive trait?​

Answers

true  mutations sometimes result in a non -adaptive trait

How can a mutation give rise to a new trait?

Genetic variations can be caused by gene variants (also known as mutations) or by a natural process in which genetic information is reorganized when a cell prepares to divide (known as genetic recombination). Different phenotypes can be introduced into an organism via genetic changes that modify gene activity or protein function.

Certain mutations, known as silent mutations, do not result in changes to the amino acid sequence of the encoded protein. Some mutations produce erroneous protein products. Mutations can add new alleles into an organism population, increasing genetic variety.

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Which most correctly describes the process of pollination in flowering plants?

The parent plant is dispersing its male offspring to mate with the female offspring of another plant.

The plant is providing pollen as food for insect visitors.

Pollen is the sperm of the parent plant and it is carried by the wind or an insect to the egg of another plant.

Pollen from the anther of one flower is carried by insects to the anther of another flower.

Answers

The statement that best describes the process of pollination in flowering plants is: ''pollen from the anther of one flower is carried by insects to the anther of another flower.''

What is pollination?

Pollination is a process in which pollen is transferred from the male reproductive organ (anther) of a flower to the female reproductive organ (stigma) of a flower of the same species, resulting in fertilization. Pollination is essential for plant reproduction and is facilitated by a variety of agents, including wind, water, birds, bees, and other animals.

In flowering plants, pollination usually occurs as follows: Pollen is produced in the anthers of the stamen, which are the male reproductive organs of a flower. Pollen grains are transferred from the anthers to the stigma, which is the female reproductive organ of a flower, either by wind, insects, or other animals.

Once the pollen grain reaches the stigma, it germinates, and a pollen tube is formed, which grows down through the style to the ovary of the flower.The pollen tube delivers sperm cells to the ovary, where they fertilize the egg cells, resulting in the formation of seeds. Finally, the ovary develops into a fruit, which contains the seeds, and the cycle begins anew.

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Which choice describes the heart?

Which choice describes the heart?

Answers

Answer:

d

Explanation:

becuase it is a organism made of tissues 1

Secretion of a lower-than-normal amount of a hormone is called __________. hyposecretion hypersecretion atrophy negative feedback

Answers

The secretion of a lower-than-normal amount of a hormone is called hyposecretion.

Hyposecretion is a medical term that describes an abnormal decrease in the production and release of hormones by the endocrine glands, resulting in lower-than-normal hormone levels in the body.

Hyposecretion of hormones might be caused by gland destruction, abnormal gland development, genetic mutations, and autoimmune disorders, among other factors, depending on the specific endocrine gland involved.

Signs and symptoms of hyposecretion of hormones can vary depending on the specific hormones that are being secreted less than usual. Some of the common symptoms may include weight loss or gain, fatigue, decreased libido, sensitivity to cold, and decreased sweating.

Treatment for hyposecretion depends on the specific hormone being secreted, and may involve hormone replacement therapy, medications, and/or surgery.

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Why would it be bad for two different populations to occupy the exact same niche at the same time of year?

Answers

Answer:

one specie's will out compete the other, forcing the other species to adapt or risk extinction

Prove the Flood a written in Genei through the ue of cientific evidence - example, rock formation

Answers

Scientific evidence such as geology, geochronology, and paleontology contradicts the idea of a global flood as described in the bible.

Additionally, the lack of geological evidence for a massive flood event that would have been necessary to cover the entire earth casts further doubt on the idea.

While some may interpret certain rock formations as evidence of a flood, these interpretations are not supported by the scientific community and are considered to be more of a religious belief rather than a scientific conclusion.

Scientific evidence, including rock formations and the fossil record, does not support a worldwide flood as described in the Bible. The scientific community generally views the story of the flood as a myth or allegory, rather than a historical event.

Complete question:

Prove the Flood a written in Genei through the use of scientific evidence - for example, rock formation

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Please help i really need a 100% on this. Remember,they may be connected to more than one

Please help i really need a 100% on this. Remember,they may be connected to more than one

Answers

Resistance to a Disease matches up with Immune System

Fetus Develops is apart of the Reproductive System

The Woman's Skin is apart of the Integumentary System

For the Flinching, it's the nervous system

Also for the Baseball player, it's the nervous system.

I hope this helps, and I also hope you get a 100% on this assignment.

In which phase do daughter nuclei began to form at the ends of the cell?
PLEAS DONT DO IT FOR POINTS I AM REALLY LOW
A. Interphase
B. Metaphase
C. Prophase
D. Cytokinesis
E. Telophase
F. Anaphase

Answers

Daughter nuclei begin to form at the ends of the cell during telophase

Which answer best describes the order of the layers of the skin from superficial to deep.
A.Epidermis, hypodermis, dermis
B.Epidermis, dermis, hypodermis
C.Hypodermis epidermis, dermis
D. None of the above

Answers

Answer:

The answer is B. Epidermis, Dermis, Hypodermis

Explanation:

Please give me brainliest if this helped

List 4 stages of making setimentary rocks

Answers

Answer:

weathering, erosion, compaction, and cementation.

PLEASE HELP ME!

Of the five characteristics of living things, which one is required for the species to survive but does not have to apply to an individual?

response to the environment

use of energy to function

reproduction

growth and change over a life cycle

Answers

Answer:

I think its 'growth and change over a life cycle', im not the best at biology  :/

What are the two things that muscle tissue can do?​

Answers

Answer: Support the weight of your body and help you move.

Explanation:

Answer:

move, be still

Explanation:

not sure if your looking for something more in depth but this is the basics

True or False?
1.Water moves through watersheds to rivers, lakes and eventually oceans.
2.Wetlands cannot filter pollution from freshwater systems.
3.Pollution can move into underground aquifers used for drinking water.
4.Ocean pollution is less harmful because it is mixed with a lot of water.
5.Ocean salinity is the same in all locations.

Answers

Answer:

True

False

True

False

False

Explanation:

now you can give the other person brainliest :D

True

False

True

False

False

       

Pollution

The stream runoff from small watersheds feeds into larger streams and rivers. The sun evaporates some water back into the air as it inflows into streams, rivers, and oceans.Wetlands' highly developed root systems hold the soil in place and filter pollutants, naturally improving water quality (including water that is eventually used for drinking).Contaminants present in these surface waters can contribute to contamination of the groundwater system. Some wells rely on artificial recharge to increase the amount of water infiltrating an aquifer, often using water from storm runoff, irrigation, industrial processes, or treated sewage.Heavy metals and other contaminants can accumulate in seafood, making it harmful for humans to consume. Microplastics can be ingested by fish and other species that filter their food out of the water.The salinity of the ocean varies from place to place, especially at the surface. Much of the ocean has salinity between 34 ppt and 36 ppt, but there are places that tend to be higher or lower.

How is pollution caused?

Pollution is caused by solid and liquid particles and certain gases that are suspended in the air. These particles and gases can come from car and truck exhaust, factories, dust, pollen, mold spores, volcanoes, and wildfires.

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How are the male and female reproductive systems similar in function, organs and organ structure, and
hormones?

Answers

Reproduction is the process through which creatures produce offspring, to put it simply. All living things share this miraculous quality, which distinguishes them from inanimate objects. However, even while a species' reproductive system.

Two different types of gametes, or sex cells, are used in human reproduction. The female reproductive system must bring together sperm, the male gamete, a secondary oocyte, the female gamete, together with the first polar body and corona radiata, in order to produce a new individual.

Similarities:

Male and female reproductive systems have some fundamental similarities and some specialized distinctions. They are similar because they are homologous, which means that the majority of the reproductive organs in both sexes grow from the same embryonic tissue. Gonads, which generate sperm, eggs, or ovums, and sex organs, are present in both systems. Additionally, the reproductive organs in both systems mature and develop sexual function during puberty as a result of gonads secreting sex hormones.

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Anyone got the answers to the UNIT: UNDERSTANDING ORGANISMS test?

Answers

The Unit 'Understanding organisms' is not found here, but an organism is a biological system composed of cells.

What is an organism?

An organism is a living entity that is defined by the presence of cells, which are the basic structural unit of life.

Organisms have the ability to respond to external stimuli, perform metabolic activities and exhibit a internal state of equilibrium (homeostasis).

Organisms can be composed of single cells (e.g., bacteria, protists) or many cells (e.g., plants and animals).

In conclusion, the Unit 'Understanding organisms' is not found here, but an organism is a biological system composed of cells.

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order of steps of muscle contraction calcium ion myosin heads pivot, and adp p are released from the myosin head

Answers

The order of steps in muscle contraction is as follows: calcium ion release and binding to troponin, myosin heads binding to actin, power stroke and pivoting of myosin heads, and release of ADP and Pi from the myosin head.

During muscle contraction, the process begins with the release of calcium ions from the sarcoplasmic reticulum in response to a neural signal. These calcium ions then bind to troponin, causing a conformational change that exposes the myosin-binding sites on actin. The myosin heads, which are part of the thick filaments, then bind to these exposed sites on actin.

This binding triggers a power stroke, where the myosin heads pivot, pulling the actin filaments towards the center of the sarcomere. This contraction is fueled by the release of ADP and inorganic phosphate (Pi) from the myosin head. After the power stroke, ATP binds to the myosin head, leading to its detachment from actin and the re-energizing of the myosin head for the next cycle of contraction.

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does sound travel at the same speed through all materials

Answers

Answer:

no

Explanation:

Sound travels fastest in liquid than in air and than in solid

Water> Air> Solid

Hope it helps :)

Answer:yes

Explanation:

what is sustainable development?
what is environmental conservation?

Give short answer​

Answers

Answer:

The long term development which is done by the preservation ,utilization and management of resources which is used by present generation and with preservation for future generation is sustainable development.

the conservation of habitat of living beings and plants is called environment conservation.

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At what terms of trade (relative price ratio) could they specialize and trade with one another so that both have more artificial hearts and pacemakers than they could have produced on their own? If Lydia can type 80 words per minute, how long will it take her to type 600 words? 1. (2 pts.) A school district is trying to determine exactly how many school buses to hire for the academic year and how much it will cost. The supply of school buses is represented by Ps = 4,000 + 8Qs and the districts demand for school buses is represented by Pd = 10,000 2Qd. a) Find the equilibrium price and quantity. b) Find the consumer and producer surpluses associated with this equilibrium. c) Now suppose that only half of the buses needed by the school district are actually available for hire. At that quantity, how much would the school district be willing to pay for each bus? Identify the new producer and consumer surpluses at this price and quantity. d) Using the prices and quantities demanded you found in parts a and c, calculate the price elasticity of demand for buses using the mid-point formula. Find the present value (at n = 0) of the following series of cash flows, with an interest rate of 6%.End of Year (EOY) 1: 300 2: 600 3: 900 4: 1200337631853005O 3100 Look at the protein below. Label the diagram on the right and show which part of the protein had hydrophobic amino acid residues and what part of the protein has hydrophilic amino acid residues. Explain how you know. Use the functions f(x) = 2x 3 and g(x) = 8x2 to calculate each operation and simplify, if possible. enter dne if the operation cannot be computed. Why would an Israeli be afraid of Hamas? what are three benefits of a smart shopping campaign? choose three. select 3 correct responses lets you reach customers across platforms uses manual signals to optimize goals uses real-time signals to help you optimize goals lets you manually set up campaigns reduces complexity of campaign management via easy integration Which one would it be?-2,1-2,-2-2,-12,2 PLEASE HELP!!! Use the number line to find the value of each point. What is the surface area of the pyramid?6 in.8 in8 in6in.8 in8 in. Read the scenario. La is researching environmental organizations and how they work together to promote climate justice. She has found the website of the Climate Action League (CAL). The website provides a lot of information on the organizations goals and strategies. However, it does not mention how other environmental groups view the Climate Action Leagues efforts or how the CAL works with other organizations.What does Las experience indicate about vertical reading?Reading vertically limits what can be learned about a source.Reading vertically does not allow for examining the parts of a source.Reading vertically ignores advertisements as an indicator of reliability Reading vertically does not consider how language can reveal bias. b Write sentences with when. Use the past simpleand past continuous.1 They/argue/ the waiter/bring/ the bill.They were arguing whenthe waiter brought the bill.2 He/fall/ off his bike /cycle /home3 The children /play/videogames/ the visitors / arrive4 We/have/a barbecueit / start / to rain5 1/finish/my report / mycomputer / crash a case-control study found a positive association between self-reported chest radiographs during pregnancy and breast cancer. Does.anyone know the answer.Will give brainliest. A sample of gas at 915.6 mmHg and occupying a volume of 12.16 Lis transferred to a smaller container of 6.55 L. What is the newpressure in kPa? Provide your answer with TWO decimals. Which philosophy promoted examinations within their civil service?- Legalism- Confucianism- Daoism- Hinduism "You want to invest your savings of $20,000 in government securities for the next 2 years. Currently, you can invest either in a security that pays interest of 8% per year for the next 2 years or in a security that matures in 1 year but pays only 6% interest. If you make the latter choice, you would then reinvest your savings at the end of the first year for another year. Why might you choose to make the investment in the 1-year security that pays an interest rate of only 6%, as opposed to investing in the 2-year security paying 8% The countries of the former Soviet Union began to industrialize in the late twentieth century and are now highly industrialized. Since the collapse of communism, their standard of living: State whether the following are Euclidean, Hyperbolic, and/orSpherical.a. The measures of the angles of a triangle add up to .b. Given a line l and a point P not on l,there is a line containing