When sequencing DNA using an automated system based on the Sanger method, which of the following is NOT required in the reactions?
Select one:
a. Primer for initiation
b. DNA template to be sequenced
c. RNA polymerase
d. Four types of ddNTPs
e. Four types of dNTPs

Answers

Answer 1

When sequencing DNA using an automated system based on the Sanger method, a. Primer for initiation is NOT required in the reactions.

The Sanger sequencing method, also called "chain termination method", is a method of determining the base sequence of DNA. This method is called the Sanger sequence because it was developed in 1977 by his two-time Nobel laureate Frederick Sanger and his colleagues.

DNA sequencing is the process of determining the sequence of the nucleotide bases (As, Ts, Cs, and Gs) of DNA fragments. With the right equipment and materials, sequencing short DNA fragments is relatively straightforward today.

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Related Questions

electron microscope magnify up to __________​

Answers

Answer:

between 1 and 50 million times

Explanation:

A light microscope can magnify things up to 2000x, but an electron microscope can magnify between 1 and 50 million times depending on which type you use! To see the results, look at the image below.

Describe the process of photosynthesis,include what is needed and what is produced.Provide an explanation as to why photosynthesis is needed for plants to maintain homeostasis.

Answers

Photosynthesis requires sunlight, carbon dioxide, and water as starting reactants. After the process is complete, photosynthesis releases oxygen and produces carbohydrate molecules, most commonly glucose. These sugar molecules contain the energy that living things need to survive.

Answer:

The process by which plants are prepared their own food by taking carbon dioxide from air and water from roots in the presence of sunlight and chlorophyll is called photosynthesis.they produced oxygen

6Co2 + 6H2o =C6H12O6 + 6O2

Explanation:

photosynthesis is ascential for their growth & development

Which of the following statements concerning chromosomes is/are true? Select all that apply.
selectk All That Apply
Chromosomes are composed of DNA and packaging proteins called histones so they may fit tightly into the nucleus of a cell.Chromosomes are composed of DNA and packaging proteins called histones so they may fit tightly into the nucleus of a cell.
Located on each chromosome are segments of DNA known as genes or units of inheritance that code for functional products.Located on each chromosome are segments of DNA known as genes or units of inheritance that code for functional products.
Homologous chromosomes contain the same genes and the same allele combination.Homologous chromosomes contain the same genes and the same allele combination.
DNA replication occurs prior to the cell cycle, in which copies of each homologous chromosomes are made and each of these copies are called sister chromatids.DNA replication occurs prior to the cell cycle, in which copies of each homologous chromosomes are made and each of these copies are called sister chromatids.
After replication, sister chromatids, which are identical copies of a chromosome, contain identical allele combinations.After replication, sister chromatids, which are identical copies of a chromosome, contain identical allele combinations.
The ultimate purpose of sexual reproduction and replicating chromosomes is to pass on a copy of a cell’s genes to the next generation of cells within the body.The ultimate purpose of sexual reproduction and replicating chromosomes is to pass on a copy of a cell’s genes to the next generation of cells within the body.

Answers

The true statements concerning chromosomes are:

1. Chromosomes are composed of DNA and packaging proteins called histones so they may fit tightly into the nucleus of a cell.

2. Located on each chromosome are segments of DNA known as genes or units of inheritance that code for functional products.

3. Homologous chromosomes contain the same genes and the same allele combination.

4. DNA replication occurs prior to the cell cycle, in which copies of each homologous chromosomes are made, and each of these copies is called sister chromatids.

5. After replication, sister chromatids, which are identical copies of a chromosome, contain identical allele combinations.

6. The ultimate purpose of sexual reproduction and replicating chromosomes is to pass on a copy of a cell's genes to the next generation of cells within the body.

1. Chromosomes are composed of DNA and histones, which are proteins that help in packaging the DNA. This packaging allows the long DNA molecule to fit tightly into the nucleus of a cell.

2. Genes are located on chromosomes. They are segments of DNA that contain instructions for building functional products like proteins. These genes serve as the units of inheritance, carrying genetic information from one generation to the next.

3. Homologous chromosomes refer to the two copies of each chromosome in a diploid cell. While they contain the same genes, they may have different alleles, or versions of those genes, which contribute to genetic variation.

4. DNA replication occurs before the cell cycle. During replication, each chromosome's DNA is copied, resulting in two identical sister chromatids attached at a central region called the centromere. This ensures that each daughter cell receives a complete set of chromosomes during cell division.

5. Sister chromatids are exact copies of each other because they are formed through DNA replication. They contain identical allele combinations since they originated from the same chromosome.

6. The purpose of sexual reproduction and the replication of chromosomes is to pass on a copy of the cell's genes to the next generation of cells within the body. This ensures the transmission of genetic information, allowing for the development of new individuals with unique combinations of genes.

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If two double-stranded DNA molecules are used at the beginning of a polymerase chain reaction (PCR) process, how many double-stranded DNA molecules can be obtained after two cycles?
a. 4
b. 32
c. 2
d. 8

Answers

During the polymerase chain reaction (PCR), a laboratory technique used to amplify DNA, two initial double-stranded DNA molecules are subjected to a series of cycles. After two cycles of PCR, the number of double-stranded DNA molecules obtained from the initial two molecules is eight. The correct answer is option d. 8.

In each cycle, the DNA is denatured into single strands and then used as a template for the synthesis of new complementary strands.

After the first cycle, each single-stranded DNA template produces two double-stranded DNA molecules. Therefore, starting with two double-stranded DNA molecules, there are four double-stranded molecules after the first cycle.

In the subsequent cycle, each of the four double-stranded molecules serves as a template, resulting in a total of eight double-stranded DNA molecules.

Thus, after two cycles of PCR, the number of double-stranded DNA molecules obtained from the initial two molecules is eight. This demonstrates the exponential amplification of DNA during the PCR process.

Therefore the correct answer is option d. 8.

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I need this answered by 11:00 please hurry

I need this answered by 11:00 please hurry

Answers

From the table, the optimum temperature for bromelain to perform its enzymatic activities is 50 degrees Celsius.

What is an optimal temperature?

When it comes to the enzymatic activities of an enzyme, the optimal temperature is the temperature at which the enzyme works best.

In other words, the optimal temperature is the temperature at which the enzyme speeds up the rate of reaction. The reaction, this time, is the breaking down of collagen from meat.

The speed or rate of any reaction is a function of time. The lower the time, the higher the rate of the reaction.

Thus, looking at the table of temperature versus time, the temperature at which the reaction time is shortest is the optimal temperature.

This temperature is 50 degrees Celsius.

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WILL MARK BRAINLIEST! In some plants, the pistils don’t form until a few days after the stamens do. How might this keep a plant from self-pollinating?

Answers

Answer:

Some plants have the ability to make the stamens mature first before the pistils to prevent itself from self-pollinating. ... If a situation as such would happen, there will be no pollen left in that flower to land on the stigma to start the fertilization of the plant.

Explanation:

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The basic objective of the layout decision is to __________. conform to manager preferences arrange activities in a line according to the sequence of operations needed minimize the number of nonadjacent loads ensure a smooth flow of work, material, people, and information through the system

Answers

The basic objective of the layout decision is to ensure a smooth flow of work, material, people, and information through the system.

This involves strategically arranging activities in a way that minimizes the number of nonadjacent loads and conforms to manager preferences, but ultimately the goal is to create an efficient and effective layout that allows for optimal productivity and workflow. Achieving this requires careful consideration of the sequence of operations needed. The basic objective of the layout decision is to ensure a smooth flow of work, material, people, and information through the system. This is achieved by organizing activities in an efficient manner, minimizing nonadjacent loads, and considering the sequence of operations required. While manager preferences may play a role, the main focus is on optimizing workflow and overall efficiency.

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A cell plate is forming across the middle of a cell and new nuclei are forming at the poles. What kind of cell is this

Answers

Answer:

Answer:It is a plant cell.

Acquired specific immunity involves the response of:_______
a. skin barriers.
b. b and t lymphocytes.
c. lysozyme.
d. mucus membranes.
e. interferon.

Answers

Acquired specific immunity involves the response of b) B and T Lymphocytes

B and T Lymphocytes are responsible for providing Acquired specific immunity to the body.

B and T Lymphocytes are formed in the bone marrow and for maturation T Lymphocytes transfers to the Thymus of the body whereas B-Lymphocytes remain confined to bone marrow for maturation and proliferation as well.

B lymphocytes provide Antibody Mediated immunity to the body which forms a part of the Acquired specific immunity of the body whereas T-lymphocytes provide Cell Mediated immunity to the body which forms a part of the Acquired specific immunity of the body.

Acquired specific immunity is acquired by the body during its lifetime on the countering of various infections and acting accordingly.

It is not present since birth hence it is not Innate.

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The most common type of rock by volume in Earth’s crust is
______________:
A. Metamorphic rock
B. Sedimentary rock
C. Divergent rock
D. Igneous rock
E. Convergent rock

Answers

The correct answer is D. Igneous rock.

Igneous rocks are the most common type of rock by volume in Earth's crust. They are formed through the solidification and crystallization of molten magma or lava. Igneous rocks can be classified into two main categories: intrusive igneous rocks and extrusive igneous rocks.

Intrusive igneous rocks form beneath the Earth's surface when molten magma cools and solidifies slowly. Examples of intrusive igneous rocks include granite and diorite. These rocks make up a significant portion of the Earth's crust.

Extrusive igneous rocks, on the other hand, form on the Earth's surface when lava erupts from volcanoes and rapidly cools and solidifies. Examples of extrusive igneous rocks include basalt and rhyolite. While these rocks may cover extensive areas on the Earth's surface, they are less voluminous compared to the intrusive igneous rocks that form beneath the surface.

Metamorphic rocks (A) are formed when existing rocks undergo changes due to high heat, pressure, or chemical processes. Sedimentary rocks (B) are formed from the accumulation and compaction of sediments over time. Divergent rock (C) and convergent rock (E) are not recognized geological terms.

Therefore, the most common type of rock by volume in Earth's crust is igneous rock (D).

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A synapse is a(n):____.

a. automatic response to sensory input. automatic response to sensory input.

b. neural cable containing many axons. neural cable containing many axons.

c. junction between a sending neuron and a receiving neuron.

d. chemical messenger that triggers muscle contractions.

Answers

A synapse is a junction between a sending neuron and a receiving neuron, facilitating the transmission of signals in the nervous system.

A synapse is a junction between a sending neuron and a receiving neuron. It is a specialized connection that allows for the transmission of signals between neurons in the nervous system. At the synapse, electrical signals known as action potentials in the sending neuron are converted into chemical signals in the form of neurotransmitters, which then traverse the synaptic gap and bind to receptors on the receiving neuron. This process is crucial for communication and information processing in the nervous system.

The synapse consists of several components, including the presynaptic terminal of the sending neuron, the synaptic cleft (the small gap between the presynaptic and postsynaptic neurons), and the postsynaptic membrane of the receiving neuron. When an action potential reaches the presynaptic terminal, it triggers the release of neurotransmitters into the synaptic cleft. These neurotransmitters bind to specific receptors on the postsynaptic membrane, initiating a cascade of events that either excite or inhibit the receiving neuron, depending on the neurotransmitter and receptor involved.

Overall, the synapse plays a vital role in the transmission of information between neurons, allowing for the integration and coordination of neural signals throughout the nervous system.

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what kind of cells perform mitoses

Answers

eukaryotic cells are the only ones that undergo mitosis, btw that link is a virus or something

energy is released from atp when question 8 options: a. a phosphate group is added. b. adenine bonds to ribose. c. atp is exposed to sunlight. d. a phosphate group is removed.

Answers

The correct answer is option (D). a phosphate group is removed. Thus energy is released from ATP when a phosphate group is removed.

By adding a phosphate group to another molecule, ATP can drive biological operations (a process called phosphorylation). For the transfer special enzymes are responsible, and to release energy from ATP the cellular process connects. Energy is needed to add a phosphate group to a molecule. Since phosphate groups are negatively charged and are organized in series in ADP and ATP, they repel one another. The ADP and ATP molecules are intrinsically unstable due to this attraction. In order to release energy, the bond between the first and second phosphates is broken. In order to release energy, the bond between the sugar and adenine is broken.

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 The correct answer is option (D). a phosphate group is removed.Subsequently energy is let out of ATP when a phosphate group is taken out.

Energy is released when an ATP molecule transfers a phosphate group to a different molecule, because the new bonds are stronger than the old bonds.

In every chemical change , exchanging one set of bonds for an additional ,

Energy is employed to break the old bonds.

Energy is released when the new bonds are formed.

If a reaction is endothermic, more energy is employed than is released. This suggests the old bonds were stronger than the new ones are.

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a membrane protein in a plant root uses atp to transport nitrate ions from a fertilizer solution in the soil and concentrates them in the cell. this process is called:

Answers

Transport is fueled by active transport, which utilizes ATP's stored energy. Integral proteins in the cell membrane, which are comparable to pumps, are used in the active transport of small molecular-sized material to move material.

Moving upwards is considered active transportation.

A cell must use energy to transfer materials against a concentration or electrochemical gradient. This is exactly what active transport systems accomplish, using energy—often in the form of ATP—to keep the proper concentrations of ions and molecules in living cells.

A symporter is a carrier protein that transports two molecules in the same direction. An antiporter is a carrier protein that transports two molecules in opposing directions. Nevertheless, some porters are able to move a single molecule across the membrane. They're known as uniporters.

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NEED HELP DUE TODAY PLZ HELP ME I AM STUCK PLZ PLZ PLZ HELP ME!!!!

Which structure represents a ribosome?

Choose 1 answer:

(Choice A)
A
Structure A

(Choice B)
B
Structure B

(Choice C)
C
Structure C

(Choice D)
D
Structure D

NEED HELP DUE TODAY PLZ HELP ME I AM STUCK PLZ PLZ PLZ HELP ME!!!!Which structure represents a ribosome?Choose

Answers

Answer:

Its D.....

I hope it helped you!!

Answer:

structure D that I am sure of

Put the following in order from the most used energy resource to the least used energy resource.
= Coal
= Natural Gas
= Nuclear
= Renewables
= Petroleum

Answers

Coal
Petroleum
Natural gas
Nuclear
Renewables

What are the likely long-term effects on ecosystems if greenhouse gas emissions combined with human over-exploitation of land continues to increase

Answers

The long-term effects of increased greenhouse gas emissions combined with human over-exploitation of land will be catastrophic for ecosystems around the world.

As temperatures rise, extreme weather events become more frequent, and the ocean acidifies, many species of plants and animals will suffer from the disruption of their habitats. Additionally, soil erosion, desertification, and pollution caused by human activities will further threaten the ability of ecosystems to recover from these disturbances.

The resulting loss of biodiversity will lead to an inability for ecosystems to adapt to the changing environment, leading to declines in ecosystem health and a further decrease in available resources for humans. In order to avert this disastrous situation, it is essential that humans take action to reduce their emissions and develop sustainable land management practices that protect the health of our planet's ecosystems.

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The presence of branchial clefts in a human embryo suggests

Answers

Humans, as an embryo, temporarily have gills.

Branchial clefts are often a common birth defect and are actually looked at as “human gills” for a small period of time until they develop further into the jaw and inner ear bones. If they do not change into those two structures, the lesions can take the form of cysts and form in the neck or just below the collarbone, causing infection that could soon seep out of the skin.

List the moon phases in order beginning and ending with the full moon: full moon, waxing gibbous, waning gibbous, waxing crescent, waning crescent, new moon, first quarter, third quarter, full moon

Answers

Answer:

Full Moon, waning gibbous Moon, last quarter Moon, waning crescent Moon, new Moon, waxing crescent Moon, first quarter Moon, waxing gibbous Moon, and finally full moon again.

Explanation:

Hope this helps :3

Answer:

new moon, waxing crescent, first quarter, waxing gibbous, full moon, waning gibbous, third quarter.

Explanation

do not allow the cell to pass onto the next phase of the cell if abnormalities are present

Answers

The cell cycle is a process that describes the cell division into two identical daughter cells. It is made up of four phases: G1, S, G2, and M.

Cells have strict regulatory mechanisms that monitor and regulate the cell cycle to ensure its proper functioning. The G1 checkpoint is the first point in the cell cycle that is regulated. The cell is prevented from entering the S phase of the cell cycle if any anomalies are present. The cell cycle is disrupted if there are any DNA damages or abnormalities, and it can cause the cell to undergo apoptosis, or programmed cell death.

Cells have a mechanism known as a checkpoint mechanism that ensures that the cycle progresses in an orderly manner. The G1 checkpoint occurs during the G1 phase of the cell cycle, and its function is to prevent cells with any genetic abnormalities from entering the S phase of the cell cycle. The S phase is the phase in which DNA replication occurs. This is a critical phase because mutations or genetic abnormalities that occur during replication can be carried forward to the daughter cells.

The G1 checkpoint checks for DNA damage, missing nutrients, and cell size, among other things, before allowing the cell to enter the S phase of the cell cycle. If any abnormalities are detected, the checkpoint will halt the cell cycle to allow time for repair or apoptosis.

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Difference in genes which pass from parents to child lead to

Answers

Differences in genes that pass from parents to child can lead to a wide range of traits and characteristics that make each individual unique.

Genetic variation, the natural variation present within a population, may be the cause of these variations.

Genetic variations due to genes in people can manifest in various ways, such as:

Physical characteristics: Physical characteristics like eye color, hair color, height, facial features, and body shape can be influenced by genetic variances.

Disease susceptibility: Genetic variations can alter a person's vulnerability to some conditions, including cystic fibrosis, sickle cell disease, and Huntington's disease.

Personality traits: According to some research, certain genes may be linked to characteristics like impulsivity, aggression, and empathy

Cognitive talents: Genetic variations can also affect cognitive skills like IQ, memory, and language proficiency.

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Salmonella bacteria, found on almost all chicken and eggs, grow rapidly in a nice warm place. If just a few hundred salmonella bacteria are left on the cutting board when a chicken is cut up, and
they get into the potato salad, the population begins compounding. Suppose the number present in the potato salad after t hours is given by f(t)=500.23.
a. If the potato salad is left out on the table, how many bacteria are present 1 hour later? b. How many were present initially?
c. How often do the bacteria double?
d. How quickly will the number of bacteria increase to 256,000?

Answers

The given function is:f(t) = 500.23(a) The given number of bacteria present in the potato salad after t hours is f(t) = 500.23.The formula for exponential growth is given by:A = PektWhere A = Final amountP = Initial amountk = rate of growtht = time elapse

ln (500.23) = ln (Pek)ln (500.23) = ln (P) + ktln (500.23) - kt = ln (P)ln (500.23) - k(1) = ln (P)P = e(ln 500.23 - k)(b) To find how many bacteria were present initially, we need to set t = 0 in the formula:f(t) = 500.23Therefore, we get:A = 500.23P = Initial amountk = rate of growtht = 0Thus, the formula becomes:500.23 = Pe0Therefore, P = 500.23(c) The time it takes for the bacteria to double can be calculated using the formula for doubling time, which is given by:T2 = ln 2/kwhere T2 is the time it takes for the bacteria to doubleWe can substitute the value of k in this formula from the formula for exponential growth as follows:A = Pektln 2 = kt2T2 = ln 2/k

Therefore,T2 = ln 2/0.000184493= 3761.61(d) We need to find how quickly the number of bacteria will increase to 256,000. We can set the final amount A equal to 256,000, the initial amount P equal to 500.23, and solve for the time t as follows:A = Pekt256000 = 500.23e0.000184493tt = ln (256000/500.23)/0.000184493t = 11.38 hours approximately.Main answer:a) After 1 hour, the number of bacteria present is f(1) = 500.23e0.000184493 × 1 = 500.81 bacteria approximately.b) The number of bacteria present initially is P = 500.23 bacteria.c) The bacteria double every 3761.61 hours.d) The number of bacteria will increase to 256,000 in about 11.38 hours. Explanation has been provided above for all the four parts of the question.

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what is shunting the blood so that it may be filtered known as?

Answers

Answer: Dialysis

Explanation: Dialysis involves either shunting the patient's blood through a special machine (haemodialysis) that helps remove the wastes while preserving water and salts, or removing wastes through fluid introduced into the abdomen (peritoneal dialysis).

Shunting the blood so that it may be filtered is known as Hemodialysis. It is a long answer. Hemodialysis is a process of removing waste and extra fluid from the blood.

Blood is filtered outside the body using a machine called a dialyzer, also known as an artificial kidney.The process of shunting blood for it to be filtered during hemodialysis is done through a vascular access, which is created by a minor surgery that connects an artery and vein, usually in the arm. The blood is pumped from the patient's bloodstream into the dialyzer, which filters the blood, and the filtered blood is returned to the patient's bloodstream.

Hemodialysis is used when the kidneys are unable to filter the blood on their own due to kidney failure. This may be caused by various factors, including diabetes, high blood pressure, glomerulonephritis, and other kidney diseases. Hemodialysis is usually done three times a week for three to five hours per session.

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4 sentence science food chain assignment (producer consumer decomposer)

4 sentence science food chain assignment (producer consumer decomposer)

Answers

What are the species used? I would write something along the lines of species #1 consumes species #2 producing waste which then is recycled into the ground. Species #3 consumes the freshly fertilized grass and is then consumed by species #4. They are all connected.

Write a short paragraph in which you use the following words correctly and show how they are related or connected. Underline each word that you used in the paragraph. matter mass volume density property

Answers

Answer:

Density the ratio of mass to that of volume .Or a measure of the amount of matter contained by a given volume.

Over 500 million years ago what was British Columbia ??

Answers

Answer:

500-million-year-old fossil found in BC is ancient ancestor of spiders and scorpions. Palaeontologists in British Columbia have found a fossil of the oldest known ancestor of scorpions and spiders.

Put these 4 parts (axon terminal, soma, axon, dendrites) in order from the part that receives signals from another neuron to the part that will pass that signal to the next neuron

Answers

Dendrites receive signals from other neurons.

Soma integrates the received signals.

Axon conducts the integrated signal over a long distance.

Axon terminal releases neurotransmitters to pass the signal to the next neuron or target cell.

So the correct order would be: dendrites -> soma -> axon -> axon terminal.

Dendrites: Dendrites receive signals from other neurons and transmit them to the cell body.

Soma (also known as the cell body): The soma integrates incoming signals and decides whether to generate an action potential

Axon: Axons are long, slender projections that carry action potentials away from the cell body.

Axon terminal (also known as the synaptic terminal): An action potential triggers the release of neurotransmitters, which transmit a signal from one neuron to the next.

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The bodies of both fishes and
reptiles are covered with Scales
but they
are into difference group why

Answers

Fishes and reptiles both have scales, but they belong to different groups because they have different evolutionary histories and biological characteristics.

Scales are protective coverings on the bodies of fishes and reptiles. Fishes and reptiles have different kinds of scales and they are classified into different groups based on their overall features and how they evolved.

     Fishes are water-dwelling animals with scales that help them to swim and helps to protect their bodies. Reptiles include animals like snakes, lizards, turtles, and crocodiles. Even though both fishes and reptiles have scales they are put into separate groups because they have different histories of evolution and different biological characteristics.

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Why do stakeholders matter as you begin to develop your team structure?.

Answers

Stake holders help you to plan the future.

Stakeholders matter as you begin to develop your team structure because they can provide valuable input into the following areas Project goals and objectives and Team composition.

Stakeholders can help you to define the goals and objectives of your project, ensuring that they are aligned with the needs of the organization.

Stakeholders can help you to identify the skills and expertise that are needed on the team, ensuring that the team is well-rounded and capable of achieving the project goals.

Stakeholders can help you to develop communication channels between the team and other stakeholders, ensuring that everyone is kept informed of progress and that any issues are addressed promptly.

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why do mutations always begin at a cellular level in an organism?

Answers

Answer:

Mutations always begin at a cellular level because the genetic material of an organism is contained within its cells. Mutations occur when there is a change in the genetic material of a cell, which can then be passed on to any offspring of the organism.

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