In PCR, a DNA polymerase is used in vitro to replicate a specific stretch of DNA within a DNA sample. However, none of the other proteins involved in DNA replication in vivo are needed. List the other proteins that are required for DNA replication in a living cell, and for each, explain why it does not need to be included in a PCR reaction.

Answers

Answer 1

Answer:

See the answer below

Explanation:

Apart from DNA polymerase, other enzymes (proteins) that are required for DNA replication in a living cell include:

HelicasePrimaseLigase; andGyrase

The helicase enzyme functions to unwind the double helix structure of the DNA during replication. This enzyme is not needed in the PCR because only a  specific stretch of a single strand DNA is needed.

The primase enzyme functions to synthesize RNA primers during DNA replication. The required primer is added directly during PCR and this makes the use of the primase enzyme unnecessary.

The ligase enzyme functions to join the Okazaki fragments of the discontinuously replicated lagging strand of the DNA during replication. There is no leading nor lagging strand in PCR and replication of the target section of the DNA is done continuously. Hence, there is no need for the ligase enzyme.

The gyrase enzyme assists in the supercoiling of DNA strands that have been initially unwound during replication. Since only a stretch of DNA is utilized for PCR, no supercoiling is needed and, hence, no need for the gyrase enzyme.


Related Questions

you are stranded on an island with a large bag of grain and two egg laying chickens. Mathematically speaking what is your best eating strategy to have enough energy for survival? use 10% energy rule to support your claim

Answers

Yes. Give the chickens all of the grain to consume, then do so. Eat the chickens eggs while feeding them all the cereal.

Are chickens able to consume cereal?

It's acceptable to occasionally eat plain grains like unsweetened Shredded Wheat, Corn Chex, and Original Cheerios. Contrarily, cereals that have been sweetened and are vividly coloured have too much sugar and artificial colouring that poultry (and probably humans) shouldn't consume frequently.

What is the preferred energy source for chickens?

All cells require the utilization of carbohydrates as a fuel source because they provide energy quickly. The majority of a chicken's diet is made up of carbohydrates. They are derived from grains of cereal. (corn, wheat, sorghum, barley, rye, millet, etc.)

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When dissolved in water, CO, creates carbonic acid which exists as dissociated ions, hydrogen
ion (H*) and carbonate (CO2) after some time.
H₂CO,
2H+ + CO²
If more carbonic acid (H₂CO₂) is added to the system, what direction will the equation shift?
Why?

Answers

If we add more carbonic acid then the equilibrium position would shift to the right

What is the direction of the equilibrium shift?

A chemical reaction at equilibrium shifts to the right to offset the change when additional reactants are added. Le Chatelier's principle, which holds that an equilibrium system will respond to a change by moving in a direction that minimizes the influence of that change, governs this movement.

We are adding more carbonic acid which is a reactant thus the equilibrium would shift to the right.

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Naive lymphocytes homing to lymphoid tissue use _____ to bind to CD34 and GlyCAM-1 on high endothelial venules.A.L-selectinB.ICAM-1C.CCL21D.CD2 (LFA-2)E.CD28

Answers

Naive lymphocytes homing to lymphoid tissue use L-selectin (A) to bind to CD34 and GlyCAM-1 on high endothelial venules (HEVs).

L-selectin, a cell adhesion molecule found on the surface of lymphocytes, plays a crucial role in lymphocyte trafficking by facilitating the initial tethering and rolling of naive lymphocytes on the endothelium of HEVs.

CD34 and GlyCAM-1 act as ligands for L-selectin, promoting lymphocyte adhesion and allowing them to enter the lymphoid tissue. This process is essential for immune surveillance, as it enables lymphocytes to encounter antigens and receive appropriate activation signals in secondary lymphoid organs.

In contrast, the other molecules listed (ICAM-1, CCL21, CD2, and CD28) serve different roles in immune cell interactions and are not directly involved in the process of naive lymphocyte homing to lymphoid tissue via binding to CD34 and GlyCAM-1 on HEVs. Hence, the correct answer is Option A.

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In the formula Ca₂SO₄, the subscript 2 means that the formula contains

Answers

Answer:

In short, the subscribt just tells you how many molecules that this compound contains. The "2" indicates that there are 2 molecules of calcium, while the "SO4" represents a sulfate ion.

Which of the following is not a physical irreversible change.

1 point

painting on a paper

bursting of balloon

cloth cut into pieces

burning of candle​

Answers

Answer:

Burning of a candle

Explanation:

All of the other things cannot be undone. the burning of a candle can. Blow the candle out and you have undone a phisical change

I hope this answer helps you :)

Answer:

I guess it is the burning of candle.

Explanation:

Melting ice and grinding wood into sawdust are examples of physical changes.

Two short-tailed cats are bred together. They produce three kittens with long tails, five short tails, and two without any tails. a. From these results, how is tail length in these cats inherited? b. Write out the genotypes paired with the matching phenotypes for the offspring to support your answer.

Answers

Answer:

a) Cats show incomplete dominance which means the kitten can be born with full length tails, short length tails and without tails.

b) The genotype of the parent will be heterozygous (Tt ) and the kittens will be:

Genotype | Fenotype

-TT | (long tail)

-Tt | (short tail)

-tt | (no tail).

what minimum radiation dose is required in order for acute radiation syndrome (ars) to occur?

Answers

The minimum radiation dose required in order for Acute Radiation Syndrome (ARS) to occur is 0.5-1.0 Gy of radiation exposure.

Acute radiation syndrome (ARS) is a result of high doses of ionizing radiation exposure in a brief period of time. It is also called radiation poisoning or radiation sickness. Acute Radiation Syndrome (ARS), also known as radiation poisoning, is a group of symptoms that occur when the human body is exposed to ionizing radiation in high doses. The symptoms of acute radiation syndrome begin when a large amount of ionizing radiation is absorbed by the body in a short period of time, usually within hours or days of exposure.

The acute radiation syndrome is divided into three stages: the prodromal stage, the latent stage, and the manifest illness stage. The severity of the symptoms and how quickly they appear are determined by the amount of radiation exposure. The minimum radiation dose required in order for Acute Radiation Syndrome (ARS) to occur is 0.5-1.0 Gy of radiation exposure.

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Which compound is released by photosynthesis and used in aerobic respiration?

carbon dioxide

water

oxygen

ATP

Answers

Question:-

Which compound is released by photosynthesis and used in aerobic respiration?

carbon dioxide

water

oxygen

ATP

Answer:-

Oxygen

Explanation:-

Photosynthesis requires carbon dioxide and releases oxygen, while cellular respiration requires oxygen and releases carbon dioxide. It is the released oxygen that we and most other organisms use.

Hi there!

The compound released by photosynthesis is

Oxygen :)

Oxygen is also used in aerobic respiration.

Hope it helps!

~GracefulGirlie

Good luck.

Which of the following has a stabilizing effect on equilibrium? A. Intraspecific competition B. Niches C. Interspecific competition D. Evolution

Answers

Answer:

The answer is option A "Intra-specific competition"

Explanation:

The equilibrium properties of an added substance multi-locus model of a quantitative attribute under recurrence and thickness subordinate determination are examined. Two contradicting transformative forces are expected to act:

1. settling determination on the attribute, which favors genotype with a middle aggregate, and

2. Intraspecific competition interceded by that quality, which favors genotype whose impact on the attribute digresses most from that of the overall genotype.

Likewise, wellness of genotypes have a recurrence free part depicting balancing out choice and a recurrence and thickness subordinate segment demonstrating competition.

Help me plz ASAP! Ty boys and girls

 Help me plz ASAP! Ty boys and girls

Answers

Answer:

Sorry im late but it would be photosynthetic cells.

5 potential negative impacts on the environment

Answers

Explanation:

Humans impact the physical environment in many ways: overpopulation, pollution, burning fossil fuels, and deforestation. Changes like these have triggered climate change, soil erosion, poor air quality, and undrinkable water.

what happens if too much water is reabsorbed into the blood from the material in the large intestine and the problems this can cause.​

Answers

Answer:

More ADH will be released, which results in water being reabsorbed and small volume of concentrated urine will be produced. If a person has consumed a large volume of water and has not lost much water by sweating, then too much water might be detected in the blood plasma by the hypothalamus.

Explanation:

If too much water is reabsorbed into the blood from the material in the large intestine, it can lead to dehydration.


What is dehydration?

The large intestine is responsible for absorbing water and electrolytes from the indigestible material that remains after digestion in the small intestine. Under normal circumstances, the amount of water that is reabsorbed is carefully regulated to ensure that the body stays hydrated and that the consistency of the stool is appropriate.

However, if too much water is reabsorbed, it can lead to dehydration, which can cause a variety of symptoms and problems. These may include:

Dry mouth and thirstDark yellow urineDry skinHeadachesFatigueConstipationDizziness or lightheadednessIncreased heart rate

Dehydration can be caused by a variety of factors, including not drinking enough fluids, sweating excessively, or having diarrhea. It is important to drink enough fluids and replace lost electrolytes to prevent dehydration. In severe cases, dehydration may require medical treatment.

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In mice, agouti coloration (A) is a dominant trait for fur color over black fur
(aA Punnett square diagram is provided. A particular generation of
offspring has a ratio of three agouti individuals to one black individual.
Which of the following genotype combinations is possible for the parent



A: aa x aa

B: AA x Ar

C:Aa x Aa

D: AA x AA

Answers

Answer:

C. Aa x Aa

Explanation:

According to this question, one gene coding for fur coloration in mice is involved. The allele for agouti coloration (A) is a dominant trait over black fur (a). This means that a heterozygote mouse will have an agouti coloration.

Based on this question, two parents were crossed to produce offsprings that have a ratio of three agouti individuals (3) to one black individual (1). Based on this phenotypic ratio, only parents that are both heterozygous for the fur color gene can produce it. That is, a cross between Aa and Aa mice parent. Hence, the genotype combination of Aa × Aa is possible for the parent to produce offsprings with 3 Agouti : 1 black.

Answer: C) Aa x Aa

I got it right!

Which description does not apply equally well to both sexual and asexual spores?
A) have haploid nuclei
B) represent the dispersal stage
C) are produced by meiosis
D) upon germination, will subsequently undergo S phase and mitosis

Answers

The description that does not apply equally well to both sexual and asexual spores is produced by meiosis. Here option C is the correct answer.

A spore is a reproductive cell that is able to grow into a new organism. Both sexual and asexual spores can be produced by a plant. Spores can be generated by meiosis (sexual spores) or mitosis (asexual spores).

The description that does not apply equally well to both sexual and asexual spores is that they are produced by meiosis. Meiosis is a process of cell division that results in the production of four haploid daughter cells from a diploid parent cell.

Sexual spores are produced by meiosis and result from the fusion of haploid gametes. Asexual spores are produced by mitosis and result in the production of genetically identical daughter cells. Therefore option C is the correct answer.

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Which of the following are reactants of photosynthesis?
O 6CO2 + 6H₂O + Energy
O C6H12O6 + 602
O CO2 + H₂O + Energy
O C6H12O6 + 02

Answers

6co2+6h2o + energy , this are the reactants

If you were to leave a pan of water outside for several days what would happen
Write a scientific report on the what would happen, and how would they relate to the tree states of water: solid, liquid and gas.

At least 3 paragraphs.​

Answers

Answer:

the water would evaporate and the water would be gone

Explanation:

hope this helps

what is the name of the syndrome that produces the xxy combination of sex chromosomes?

Answers

The syndrome that produces the XXY combination of sex chromosomes is called Klinefelter syndrome.

Klinefelter syndrome is a chromosomal disorder that occurs in males and is characterized by the presence of an extra X chromosome, resulting in a 47,XXY karyotype. Typically, males have an XY sex chromosome configuration. The additional X chromosome in individuals with Klinefelter syndrome can lead to various physical and developmental differences, such as infertility, reduced testosterone production, gynecomastia (enlarged breasts), tall stature, and learning difficulties. Early diagnosis and appropriate management can help address the potential challenges associated with this syndrome.

 

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Which is a positive effect of the technological advance of going from a compass to a gps

Answers

Just like a compass, a modern day gps will say whether you're going north, east, south, or west, however they also tell your exact location and the location that you need to go too.

what are the main difference between animal and plant cells??

How do these differences influence the internal structure of these cell​

Answers

The main difference is that Animal Cells do not have a cell wall, plants do. there's obviously more but I believe that's the main difference

A cell membrane and a cell wall are features of plant cells. The cell membrane is surrounded by the cell wall in plants. The plant cell's distinctive rectangular shape is due to this. Plant cells lack the centrosome and lysosome, while animal cells only have a cell membrane and no cell wall, giving them a circular shape. Unlike animal cells, plant cells have a cell wall, chloroplasts and other specialized plastids, and a large central vacuole.

does photosynthesis occur in mushroom.why? ​

Answers

Answer:

no

Explanation:

because it is fungi and it doesnt have chloroplast (which is needed for photosynthesis)

Which of Newton’s laws explains why the balls did not remain in the container at the end of the ramp?
Newton’s Law of inertia
Newton’s Law of force, mass, acceleration
Newton’s law of Action/ Reaction
Newton’s law of gravity

Which of Newtons laws explains why the balls did not remain in the container at the end of the ramp?Newtons

Answers

I’m pretty sure it’s Newton’s law of Action/Reaction. “For every action, there is an equal and opposite reaction.”

Answer:Newton's first law

Explanation:

Friction and gravitational pull are common unbalanced forces that change the motion of objects on the Earth. Friction acts on objects to slow them down, such as a ball rolling across something like a carpet or sand.

Select the correct answer from each drop-down menu.
Cancer is a disease that involves uncontrolled cell division caused by a genetic mutation. It can occur in almost any region in the body. So, cancer is essentially uncontrolled ______ of _____
Blank 1:
meiosis
mitosis
regeneration
Blank 2:
embryonic stem cells
gamete cells
specialized cells

Answers

Answer:

Cancer is a disease that involves uncontrolled cell division caused by a genetic mutation. It can occur in almost any region in the body. So, cancer is essentially uncontrolled mitosis of specialized cells

Explanation:

It can occur in almost any region in the body. So, cancer is essentially uncontrolled mitosis of specialized cells. The correct options are b and c respectively.

What is cancer?

Cancer is a medical condition in which cells in one portion of the body expand and multiply inconsolably.

Cancerous cells have the ability to invade and destroy healthy tissue, including organs. Cancer can start in one part of the body and spread to other parts. This is referred to as metastasis.

Cancer is caused by changes in genes, which are the basic physical units of inheritance. Genes are organized in chromosomes, which are long strands of tightly packed DNA.

Cancer is a genetic disease, which means that it is caused by changes in genes that regulate how our cells function, particularly how they grow and divide mitotically.

Thus, the correct options are b and c respectively.

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What is the process of action potential conduction along the axon called?

Answers

The process of action potential conduction along the axon is called propagation of action potential

The conduction of action potential along the axon body is propagated through its axon terminal. A change in charge(positive to negative or vice versa) occurs via membranes of axon(myelinated or non-myelinated) that causes the impulse to transcend and move along the axon.

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What are the 4 phases of mitosis and what happens in each?

Answers

Mitosis is the process of cell division that occurs in somatic cells, resulting in the formation of two identical daughter cells. It can be divided into four main phases: prophase, metaphase, anaphase, and telophase.

1. Prophase: During this phase, chromatin fibers condense and become visible as chromosomes. The nuclear envelope breaks down, and the centrosomes move to opposite poles of the cell, forming the spindle fibers. The chromosomes attach to the spindle fibers by their kinetochores.

2. Metaphase: During this phase, the chromosomes line up along the metaphase plate, which is the imaginary plane that bisects the cell. The spindle fibers attach to the kinetochores on the chromosomes, and the tension on the fibers aligns the chromosomes in a single line.

3. Anaphase: During this phase, the sister chromatids separate and are pulled towards opposite poles of the cell by the spindle fibers. The cell elongates as the non-kinetochore spindle fibers push against each other, and the cell prepares to divide into two daughter cells.

4. Telophase: During this phase, the nuclear envelope reforms around the two sets of chromosomes, and the chromosomes begin to unwind and decondense back into chromatin fibers. The spindle fibers disassemble, and the cell undergoes cytokinesis, the process of dividing the cytoplasm and forming two identical daughter cells. In animal cells, a cleavage furrow forms, while in plant cells, a cell plate forms to divide the cytoplasm.

These four phases of mitosis ensure that each daughter cell receives an identical set of chromosomes, and the process of cell division is critical for the growth and repair of tissues in multicellular organisms.

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In an energy pyramid, up to 10% of energy is transferred between trophic levels. Suppose the producer level has 50,000 J. Approximately how much energy is available to the next level, the primary consumer, in this energy pyramid?

Answers

Answer: 5,000

Explanation:So it is the 10% law, this is an example 10,000 for the first animal, 1,000 for the second and 100 for the third. So for 50,000 it would be 5,000 for the next

Can anyone help me ?

Can anyone help me ?

Answers

Yes, the metaphor and the cycle

In an experiment in my laboratory, I used siRNA to decrease the amounts of a HDAC that removes the H3K9ac mark. What observations would I expect in terms of changes in gene expression?

Answers

If you have used siRNA to decrease the amounts of a HDAC that removes the H3K9ac mark, you may expect to see changes in gene expression, as the H3K9ac mark is associated with increased transcriptional activity.

Overall, the specific changes in gene expression that you observe will depend on the specific HDAC that you targeted, the cell type that you are working with, and the genes that are regulated by H3K9ac in that cell type.

Specifically, when the HDAC is inhibited, the H3K9ac mark may become more abundant, leading to increased acetylation of histones and an open chromatin structure.

This open chromatin structure may allow for greater accessibility of transcription factors and RNA polymerase to the DNA, resulting in increased transcriptional activity of genes that are regulated by H3K9ac.

Depending on the specific genes that are regulated by H3K9ac, you may observe either an upregulation or downregulation in gene expression.

For example, if the HDAC you targeted is involved in the repression of genes that are normally activated by H3K9ac, then you may expect to see an upregulation of those genes.

Conversely, if the HDAC you targeted is involved in the activation of genes that are normally repressed by H3K9ac, then you may expect to see a downregulation of those genes.

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What advantage do invasive species of plants have over native species?.

Answers

They are better competitors because they emerge earlier in the spring, grow rapidly, and are impacted by few, if any, natural predators. When invasive plants are allowed to grow unchecked, many native plants and the wildlife species that rely upon them suffer.

the taste receptor that amino acids such as glutamic acid activate is

Answers

The taste receptor that amino acids such as glutamic acid activate is the umami receptor.

Umami is one of the five basic tastes alongside sweet, sour, bitter, and salty. It is a Japanese word that means "deliciousness" or "pleasant savory taste."Umami taste is triggered by the presence of the amino acid glutamic acid or its salts, known as glutamates.

Other amino acids such as aspartic acid and nucleotides like inosinate and guanylate can also enhance the umami flavor. The umami receptor is a G protein-coupled receptor that is found on the surface of specialized taste cells on the tongue.

When umami compounds bind to the receptor, it triggers a series of biochemical reactions that send a signal to the brain, resulting in the perception of the umami taste. Overall, the umami taste contributes to the complexity and enjoyment of food and is commonly found in savory dishes such as meat, broth, and cheese.

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What principle can be applied to all chemical reactions?

A) conservation of mass
B) reactants formed from products
C) energy loss theory
D) coefficient principle

Answers

A. Conservation of mass
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